Aims and Objective: The present study was designed to evaluate the xanthine oxidase (XO)
inhibitory and antioxidant activities of 30 bioactive compounds present in edible food plants
for the possible treatment of hyperuricemia.
Materials and Methods: The XO inhibitory, SO and DPPH radical scavenging activities of
selected dietary polyphenols were determined by using colorimetric assays. The molecular
docking analysis was performed to evaluate the insight into inhibitory mode of action of
bioactive compounds against XO.
Results: The results show that apigenin, galangin, kaempferol, quercetin, genistein and
resveratrol potently inhibit XO enzyme among all tested compounds. Flavonoids exhibit
higher, anthocyanins and hydroxycinnamic acids moderate, maslinic acid, ellagic acid,
salicylic acid, -gingerol and flavan-3-ols showed weak XO inhibitory activity. The results
of molecular docking study revealed that these bioactive compounds bind with the active site
of XO and occupy the active site which further prevents the entrance of substrate and results
in the inhibition of XO.
Conclusion: Inhibition of XO gives a robust biochemical basis for management of
hyperuricemia, gout and other associated diseases via controlling uric acid synthesis.