Title:T-cell Epitope-based Vaccine Design for Nipah Virus by Reverse Vaccinology Approach
VOLUME: 23 ISSUE: 8
Author(s):Praveen K.P. Krishnamoorthy*, Sekar Subasree, Udhayachandran Arthi, Mohammad Mobashir, Chirag Gowda and Prasanna D. Revanasiddappa
Affiliation:Department of Biotechnology, Sri Venkateswara College of Engineering, Pennalur, Sriperumbudur 602117, Tamilnadu, Department of Biotechnology, Sri Venkateswara College of Engineering, Pennalur, Sriperumbudur 602117, Tamilnadu, Department of Biotechnology, Sri Venkateswara College of Engineering, Pennalur, Sriperumbudur 602117, Tamilnadu, Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institute, Novels vag 16, 17165 Solna, Stockholm, Department of Biotechnology, Siddaganga Institute of Technology, Tumkuru 572103, Karnataka, Department of Biotechnology, Siddaganga Institute of Technology, Tumkuru 572103, Karnataka
Keywords:Nipah, vaccination, immunogenicity, conservation, toxicity, docking, simulation.
Abstract:
Aim and Objective: Nipah virus (NiV) is a zoonotic virus of the paramyxovirus family
that sporadically breaks out from livestock and spreads in humans through breathing resulting in an
indication of encephalitis syndrome. In the current study, T cell epitopes with the NiV W protein
antigens were predicted.
Materials and Methods: Modelling of unavailable 3D structure of W protein followed by docking
studies of respective Human MHC - class I and MHC - class II alleles predicted was carried out for
the highest binding rates. In the computational analysis, epitopes were assessed for
immunogenicity, conservation, and toxicity analysis. T – cell-based vaccine development against
NiV was screened for eight epitopes of Indian - Asian origin.
Results: Two epitopes, SPVIAEHYY and LVNDGLNII, have been screened and selected for
further docking study based on toxicity and conservancy analyses. These epitopes showed a
significant score of -1.19 kcal/mol and 0.15 kcal/mol with HLA- B*35:03 and HLA- DRB1 * 07:03,
respectively by using allele - Class I and Class II from AutoDock. These two peptides predicted by
the reverse vaccinology approach are likely to induce immune response mediated by T – cells.
Conclusion: Simulation using GROMACS has revealed that LVNDGLNII epitope forms a more
stable complex with HLA molecule and will be useful in developing the epitope-based Nipah virus
vaccine.
