Biochemical and Computational Approaches for the Large-Scale Analysis of Protein Arginine Methylation by Mass Spectrometry

Author(s): Daniele Musiani, Enrico Massignani, Alessandro Cuomo, Avinash Yadav, Tiziana Bonaldi*

Journal Name: Current Protein & Peptide Science

Volume 21 , Issue 7 , 2020

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Graphical Abstract:


The absence of efficient mass spectrometry-based approaches for the large-scale analysis of protein arginine methylation has hindered the understanding of its biological role, beyond the transcriptional regulation occurring through histone modification. In the last decade, however, several technological advances of both the biochemical methods for methylated polypeptide enrichment and the computational pipelines for MS data analysis have considerably boosted this research field, generating novel insights about the extent and role of this post-translational modification.

Here, we offer an overview of state-of-the-art approaches for the high-confidence identification and accurate quantification of protein arginine methylation by high-resolution mass spectrometry methods, which comprise the development of both biochemical and bioinformatics methods. The further optimization and systematic application of these analytical solutions will lead to ground-breaking discoveries on the role of protein methylation in biological processes.

Keywords: Protein arginine methylation, mass spectrometry, proteomics, computational pipelines, methyl-peptide enrichment, protein arginine methyltransferases.

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Article Details

Year: 2020
Published on: 22 September, 2020
Page: [725 - 739]
Pages: 15
DOI: 10.2174/1389203721666200426232531
Price: $65

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