Background: Drug-protein complexes is one of the crucial factors when analyzing the
pharmacokinetics and pharmacodynamics of a drug because they can affect the excretion, distribution,
metabolism and interaction with target tissues.
Objectives: The aim of this study was to investigate the interaction of human hemoglobin (Hb) and
angiotensin I converting enzyme inhibitory peptide (ACEIP) in the absence and presence of different-
frequency electromagnetic fields (EMF).
Methods: Various spectroscopic methods like fluorescence spectroscopy, ultraviolet, circular
dichroism and conductometry techniques were applied to investigate Hb-ACEIP interaction in the
absence and presence of EMF.
Result: The presented spectroscopic studies indicated that EMF changed the interaction between
Hb and ACEIP. The a-helix content of Hb decreased upon binding to ACEIP and conductivity of
the solution enhanced upon binding. Based on Stern-Volmer equations, it could be stated that the
Hb-ACEIP affinity was higher in the presence of EMF.
Conclusion: It can be concluded that for patients who use the drug to control blood pressure, a
low-frequency electromagnetic field would have a positive effect on the uptake of the drug.