Title:Difference of Chemical Compositions in Fu Zheng Fang with Different Dosage Forms Based on HPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis
VOLUME: 17 ISSUE: 6
Author(s):Yujiao Hua*, Fen Xie and Shijun Zhou
Affiliation:Department of Clinical Pharmacy, The Affiliated Hospital of Jiangnan University, Wuxi 214000, Department of Clinical Pharmacy, The Affiliated Hospital of Jiangnan University, Wuxi 214000, Department of Clinical Pharmacy, The Affiliated Hospital of Jiangnan University, Wuxi 214000
Keywords:Fu Zheng Fang, HPLC-Q-exactive orbitrap/MS, differential chemical compositions, TCM decoction pieces, TCM
formula granules.
Abstract:Background: Fu Zheng Fang (FZF) is an important Chinese medicine prescription for tumor
treatment in hospitals, which has two different types, traditional Chinese medicine (TCM) decoction
pieces and TCM formula granules.
Objective: This study aimed to determine the effective composition of the drug FZF.
Methods: In this research, FZF decoction pieces and FZF formula granules were collected and their
composition, determined by HPLC-Q-Exactive Orbitrap/MS, and multivariate statistical analysis, was
applied to distinguish differential metabolite patterns between two groups.
Results: A clear cut difference in the composition of the two groups was observed. 124 differential
chemical compositions could be identified in the positive mode, while 59 differential chemical compositions
could be identified in the negative mode. The differential chemical compositions were mainly
concentrated in flavonoids, organic acids, fatty acids, amino acid compounds, and presenting different
change rules, mainly involved in two metabolic pathways; the flavonoid biosynthesis, and flavone and
flavonol biosynthesis.
Conclusion: This study provides basic information that may be of use in the formulation of the drug in
different dosages and in the examination of their efficacy.
