Anodic Adsorptive Stripping Voltammetric Determination of Rafoxanide on Glassy Carbon Electrode

(E-pub Ahead of Print)

Author(s): Abd-Elgawad Radi*, Hassan El-samboskany

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

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Background: Rafoxanide (RFX) is an active agent against Fasciola hepatica, but it is prohibited for treatment of dairy animals whose milk is provided for human consumption.

Objective: A reliable, fast, and simple method needs to be developed to monitor RFX residues in milk samples before distribution to consumers.

Methods: In this work, the electrochemical oxidation of RFX was studied at glassy carbon electrodes (GCE) in Britton-Robinson buffer (BR) solutions over the pH range 2.0-12.0 using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The oxidation of the drug was accomplished in a single irreversible, adsorption-controlled step within the pH range 4.0-9.0. Therefore, the application of GCE for a sensitive and selective quantification of RFX by adsorptive stripping voltammetry was reported. The accumulation of the analyte was performed in Britton–Robinson buffer (pH 5.0) at a potential of -0.3 V (vs. Ag-AgCl-KClsat) for 300 s and the measurement was carried out, after medium exchange, in BR solution of pH 7.0 using DPV.

Result and Conclusion: This format was satisfactorily applied for the determination of RFX in bovine milk. Limit of detection (LOD) of 1.25 µg kg -1 of milk and mean recoveries of 97.8 to 107.5% were achieved.

Keywords: Rafoxanide, anodic adsorptive stripping voltammetry, bovine milk, glassy carbon electrode, cyclic voltammetry, differential pulse voltammetry.

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(E-pub Ahead of Print)
DOI: 10.2174/1386207323666200422083339
Price: $95