Anodic Adsorptive Stripping Voltammetric Determination of Rafoxanide on Glassy Carbon Electrode

Author(s): Abd-Elgawad Radi*, Hassan El-samboskany

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 23 , Issue 10 , 2020

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Aims and Objective: The development of easy, accurate, reliable technique which is characterized by low cost, minimal sample pre-treatment, and short analysis time to monitor RFX residues in milk samples before distribution to consumers.

Background: Literature survey reveals several analytical methods, including high-performance liquid chromatography (HPLC), ultra-performance liquid chromatography (UPLC) and thin-layer chromatography (TLC)-densitometry. These methods are time consuming, require additional steps like preconcentration or multisolvent extraction, trained technicians, and expensive instruments.

Materials and Methods: The electrochemical analysis of RFX was effectively established by the adsorptive stripping method on GCE due to the effective interfacial accumulation of RFX on the electrode surface. The RFX adsorptive accumulation is followed by electrochemical measurement of the accumulated analyte.

Results: The electrochemical oxidation of RFX was studied at glassy carbon electrodes (GCE) in Britton-Robinson buffer (BR) solutions over the pH range from 2.0-12.0 using cyclic and differential pulse voltammetry (DPV). The oxidation of the drug was accomplished in a single irreversible, adsorption-controlled step within the pH range 4.0-9.0. Therefore, the application of GCE for a sensitive and selective quantification of RFX by adsorptive stripping voltammetry was reported. This format was satisfactorily applied for the determination of RFX in bovine milk. Limit of detection (LOD) of 1.25 μg kg-1 of milk and mean recoveries of 97.8 to 107.5% were achieved.

Conclusion: The proposed method might be competitive with the HPLC techniques. The detection limit found for RFX on GCE for milk samples, after medium exchange, was well below the MRLs, the maximum concentration of a veterinary drug residue legally permissible in food, are proposed by the European Medicines Agency.

Keywords: Rafoxanide, anodic adsorptive stripping voltammetry, bovine milk, glassy carbon electrode, cyclic voltammetry, differential pulse voltammetry.

Sweetman, S.C. Martindale: The Complete Drug Reference, 34th ed; Pharmaceutical Press: London, UK, 1999.
Lanusse, C.E.A.; Luis, I. Virkel, Guillermo L. Anticestodal and antitrematodal drugs. Rafoxanide.In: Veterinary Pharmacology and Therapeutics; Riviere, J.E.; Papich, M.G., Eds.; John Wiley & Sons, 2018.
Blanchflower, W.J.; Kennedy, D.G.; Taylor, S.M. Determination of rafoxanide in plasma using high performance liquid chromatography (hplc) and in tissue using hplc-thermospray mass spectrometry. J. Liq. Chromatogr., 1990, 13(8), 1595-1609.
Power, C.; Danaher, M.; Sayers, R.; O’Brien, B.; Whelan, M.; Furey, A.; Jordan, K. Investigation of the persistence of rafoxanide residues in bovine milk and fate during processing. Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess., 2013, 30(6), 1087-1095.
Yeung, H.S.; Ching, W.H.; Lai, S.S.L.; Lee, W.O.; Wong, Y.T. Quantitative analysis of closantel and rafoxanide in bovine and ovine muscles by high-performance liquid chromatography with fluorescence detection. AOAC, 2010, 93(5), 1672-1677.
Yeung, H.S.; Lee, W.O.; Wong, Y.T. Screening of closantel and rafoxanide in animal muscles by HPLC with fluorescence detection and confirmation using MS. J. Sep. Sci., 2010, 33(2), 206-211.
Saad, A.S.; Attia, A.K.; Alaraki, M.S.; Elzanfaly, E.S. Comparative study on the selectivity of various spectrophotometric techniques for the determination of binary mixture of fenbendazole and rafoxanide. Spectrochim. Acta A, 2015, 150, 682-690.
Benchaoui, H.; McKellar, Q. Determination of rafoxanide and closantel in ovine plasma by high performance liquid chromatography. Biomed. Chromatogr., 1993, 7(4), 181-183.
Fink, D.W. Spectrophotometric quantification of the salicylanilide anthelmintic rafoxanide based on the charge-transfer absorbance of its iron(III) complex. Anal. Chim. Acta, 1981, 131(C), 281-285.
Talley, C.P.; Trenner, N.R.; Downing, G.V.; VandenHeuvel, W.J.A. Gas chromatographic determination of rafoxanide [3-chloro-4-(4-chlorophenoxy)-3,5-diiodosalicylanilide] in plasma by electron capture detection of its trimethylsilyl derivative. Anal. Chem., 1971, 43(11), 1379-1382.
Saad, A.S.; Hamdy, A.M.; Salama, F.M.; Abdelkawy, M. Validated UPLC and TLC-densitometry stability indicating methods for the determination of rafoxanide in the presence of its degradation products. J. Chromatogr. Sci., 2016, 54(9), 1661-1669.
Alghamdi, A.F. High sensitivity determination of atorvastatin calcium in pharmaceuticals and biological fluids using adsorptive anodic stripping voltammetry onto surface of ultra-trace graphite electrode. Curr. Anal. Chem., 2018, 14(2), 92-100.
Allahverdiyeva, S.; Talay Pınar, P.; Keskin, E.; Yunusoğlu, O.; Yardım, Y.; Şentürk, Z. Adsorptive stripping voltammetric determination of higenamine on a boron-doped diamond electrode improved by the use of an anionic surfactant. Sensor Actuat. B Chem., 2020, 303, 127174.
Radi, A.E.; Eissa, A.; Wahdan, T. Voltammetric behavior of mycotoxin zearalenone at a single walled carbon nanotube screen-printed electrode. Anal. Methods, 2019, 11(35), 4494-4500.
Santos, A.M.; Wong, A.; Cincotto, F.H.; Moraes, F.C.; Fatibello-Filho, O. Square-wave adsorptive anodic stripping voltammetric determination of norfloxacin using a glassy carbon electrode modified with carbon black and CdTe quantum dots in a chitosan film. Mikrochim. Acta, 2019, 186(3), 148.
Temerk, Y.; Ibrahim, H.; Schuhmann, W. Simultaneous anodic adsorptive stripping voltammetric determination of luteolin and 3-hydroxyflavone in biological fluids using renewable pencil graphite electrodes. Electroanalysis, 2019, 31(6), 1095-1103.
Van der Linden, W.; Dieker, J.W. Glassy carbon as electrode material in electro-analytical chemistry. Anal. Chim. Acta, 1980, 119(1), 1-24.
Laviron, E. General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical systems. J. Electroanal. Chem., 1979, 101(1), 19-28.
Zoski, C.G. Handbook of Electrochemistry; Elsevier, 2006.
Abderezzak, B. 2-Charge Transfer Phenomena.In: Introduction to Transfer Phenomena in PEM Fuel Cell; Abderezzak, B., Ed.; Elsevier, 2018, pp. 53-83.
Compton, R.G.; Banks, C.E. Understanding Voltammetry; World Scientific: New Jersey, 2018, p. 157.
Bard, A.J.; Faulkner, L.R.; Zoski, C.G.; Leddy, J. Electrochemical Methods: Fundamentals and Applications; John Wiley: New York, 2001.
Radi, A-E.; El-Ghany, N.A.; Wahdan, T. Anodic voltammetric methods for determination of the antiparasitic drug nitazoxanide in bulk form, pharmaceutical formulation, and its metabolite tizoxanide in human serum. Monatsh. Chem., 2012, 143(5), 697-702.
Alemu, H.; Khoabane, N.M.; Tseki, P.F. Electrochemical oxidation of niclosamide at a glassy carbon electrode and its determination by voltammetry. Bull. Chem. Soc. Ethiop., 2003, 17(1), 95-106.
Dede, E.; Saǧlam, Ö.; Dilgin, Y. Sensitive Voltammetric Determination of Niclosamide at a disposable pencil graphite electrode. Electrochim. Acta, 2014, 127, 20-26.
Lu, L.; Seenivasan, R.; Wang, Y-C.; Yu, J-H.; Gunasekaran, S. An electrochemical immunosensor for rapid and sensitive detection of mycotoxins fumonisin B1 and deoxynivalenol. Electrochim. Acta, 2016, 213, 89-97.
Commission Implementing Regulation (EU) No 681/2014 of 20 June 2014 amending Regulation (EU) No 37/, 2010.

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Article Details

Year: 2020
Page: [1002 - 1009]
Pages: 8
DOI: 10.2174/1386207323666200422083339
Price: $65

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