Background: Polyphenolic compounds are known to provide health benefits and protect
against degenerative chronic diseases. Utilization and identification of foods with a high
content of these compounds are gaining greater attention nowadays.
Objective: The present study reports the total phenolic content (TPC), polyphenolic composition
and antioxidant activity (DPPH, FRAP and TEAC) of 10 commonly consumed leafy vegetables
growing in Bangladesh.
Materials and Methods: The samples were collected from different locations of Bangladesh and
mixed together to ensure sample representativeness. Folin-Ciocalteu method was used for the
analysis of TPC, and quantification of polyphenolic components was done by high-performance
liquid chromatography (HPLC- DAD). Additionally, antioxidant activities of the selected vegetables
were also analysed by utilizing DPPH, FRAP & TEAC.
Results and Discussion: TPC ranged from 23.64 ± 1.20 to 45.59 ± 3.04 mg gallic acid equivalent
(GAE)/g freeze-dried sample (fds). The polyphenolic spectrum ranged from 0.30 ± 0.02 to
647.42 ± 147.12 mg/100 g fds; quantity and spectrum of which varied in the vegetables. Among
the studied vegetables, Centella asiatica contained the highest amount of TPC (45.59 ± 3.04 mg
GAE/g fds) and also exhibited high antioxidant capacities, as documented by DPPH, FRAP and
TEAC assays. Moreover, Principal component analysis (PCA) of investigated variables clearly
separated Centella asiatica from other samples.
Conclusion: Phenolic compounds being strong antioxidants reduce the risk of chronic diseases
and the finding of this study would aware the people to take vegetables rich in phenolics. It
would also fill up the data gap in the existing food composition table of Bangladesh.