Background: Evaluation of the toxic effects of nanoparticle-drug in vitro is an important
step in the design of new pharmaceutical formulations. Rapid results, reduced cost and easy
handling makes cell culture models first line in initial toxicological assessment of nanodrug preparations.
Objective: To evaluate the in vitro cytotoxicity of oxytetracycline loaded calcium carbonate
aragonite nanoparticle in normal mouse fibroblast (NIH3T3) cell line.
Method: NIH3T3 cells were exposed to varying concentrations (6.25 - 100µg/mL) of
calcium carbonate aragonite nanoparticle (CS-CaCO3NP), oxytetracycline loaded calcium
carbonate aragonite nanoparticle (OTC-CS-CaCO3NP) and oxytetracycline (OTC) in 96 well
plates for 24, 48 and 72 hours. Cell viability was determined by MTT and trypan blue assays.
Result: Both assays show that CS-CaCO3NP and OTC-CS-CaCO3NP had higher cell
viability values compared to OTC.
Conclusion: Encapsulating OTC into CS-CaCO3NP reduced its cytotoxicity to NIH3T3
cells using both MTT and trypan blue assay.