Aim and Objective: Lupus nephritis (LN) is one of the major complications of systemic
lupus erythematosus (SLE). The specific mechanisms of pathogenesis, aggravation, and remission
processes in LN have not been clarified but is of great need in the clinic. Using isobaric tags for
relative and absolute quantitation (iTRAQ) technology to screen the functional proteins of LN in
mice. Especially under intervention factors of lipopolysaccharide (LPS) and dexamethasone.
Methods: Mrl-lps mice were intervened with LPS, dexamethasone, and normal saline (NS) using
intraperitoneal injection, and c57 mice intervened with NS as control. The anti-ANA antibody
enzyme-linked immunosorbent assay (ELISA) was used to verify disease severity. Kidney tissue is
collected and processed for iTRAQ to screen out functional proteins closely related to the onset
and development of LN. Western blot method and rt-PCR (real-time Polymerase Chain Reaction)
were used for verification.
Results: We identified 136 proteins that marked quantitative information. Among them, Hp,
Igkv8-27, Itgb2, Got2, and Pcx proteins showed significant abnormal manifestations.
Conclusion: Using iTRAQ methods, the functional proteins Hp, Igkv8-27, Itgb2, Got2, and Pcx
were screened out for a close relationship with the pathogenesis and development of LN, which is
worth further study.