Antimicrobial and Antibiofilm Activity of Lys-[Trp6]hy-a1 Combined with Ciprofloxacin Against Gram-Negative Bacteria

Author(s): Victor Alves Carneiro*, Simone Torres de Oliveira, Rondinely Lima Silva, Humberlania de Sousa Duarte, Maria Laína Silva, Maria Nágila Carneiro Matos, Rafaela Mesquita Bastos Cavalcante, Ciro Siqueira Figueira, Esteban Nicolás Lorenzón, Eduardo Maffud Cilli, Rodrigo Maranguape Silva da Cunha

Journal Name: Protein & Peptide Letters

Volume 27 , Issue 11 , 2020


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Abstract:

Background: Ciprofloxacin (Cip) is the most commonly used quinolone in clinical practice; however large-scale use has favored the increase of multiresistant pathogenic microorganisms. Antimicrobial peptides (AMPs) appear to be a promising alternative in potentiating these conventional drugs.

Objective: The aim of this study was to evaluate the effect of the peptide Lys-[Trp6]hy-a1 (lys-a1) on the antimicrobial and antibiofilm activity of ciprofloxacin against clinically relevant gram-negative bacteria.

Methods: The antimicrobial effects of Cip and lys-a1 were assessed by determining the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). The synergistic action of Cip and lys-a1 was determined by checkerboard assay. The time-kill curve was constructed for the Cip/lys-a1 combination against Pseudomonas aeruginosa ATCC 9027. The antibiofilm activity of this combination was analyzed by crystal violet, colony-forming unit count and atomic force microscopy (AFM).

Results: The data demonstrated that lys-a1 was able to inhibit planktonic growth of strains of P. aeruginosa and Klebsiella pneumoniae both at 125 μg/mL. The fractional inhibitory concentration index (FICi) showed a synergistic effect between Cip and lys-a1 against P. aeruginosa, decreasing the MICs of the individual antimicrobial agents by 4- and 8-fold, respectively. This effect was also observed for the death kinetics and antibiofilm activity. Analysis of the early biofilms (6 h) as well as isolated cells by AFM images evidenced the cell perturbation caused by Cip/lys-a1 treatment.

Conclusion: These data suggest that lys-a1 has biotechnological potential as a therapeutic tool for the treatment of infections caused by clinically relevant microorganisms, especially P. aeruginosa.

Keywords: Lys-a1, antimicrobial peptide, Pseudomonas aeruginosa, ciprofloxacin, synergism, antibiofilm activity.

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Article Details

VOLUME: 27
ISSUE: 11
Year: 2020
Published on: 16 November, 2020
Page: [1124 - 1131]
Pages: 8
DOI: 10.2174/0929866527666200416145549
Price: $65

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