Metabolic Changes in Hyperlipidemic Rats After the Administration of Xuezhikang

Author(s): Ke Su, Bingbao Chen, Xiaoting Tu, Luxin Ye, Xiaojie Lu, Zheng Yu, Xianqin Wang*, Xuezhi Yang*

Journal Name: Current Pharmaceutical Analysis

Volume 17 , Issue 6 , 2021


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Abstract:

Background: Xuezhikang capsule, which contains cholesterol synthase inhibitors and a large number of natural statins, is put in the clinical application of lipid-lowering and so on. However, the specific use of dose, lipid-lowering effect and the relationship between metabolites are to be further studied.

Introduction: Metabonomics is the study of the relationship between the change of quantity and physiological changes from metabolites. At present, metabolomics has been widely used in drug development and testing. In this study, we developed a metabolomic method based on gas chromatographymass spectrometry (GC-MS) to find out hyperlipemia-related substances, and study the lipid-lowering mechanism of Xuezhikang.

Method: Fifty SD rats (220 ± 20 g) were given a high-fat diet. After four-weeks modeling, they were randomly divided into semi-control groups, high fat group, simvastatin intervention group and Xuezhikang intervention group (0.23, 0.69, 1.15 mg/kg, low, medium, high), each dosage in eight rats. The control group (rest eight rats) were given a normal diet, and no specific treatment. The rats were sacrificed at the end of the experiment.

Result: The biochemical and body weight indexes of the normal control group and the high fat group were significantly different (P <0.05), which indicated that the model of hyperlipidemia was established success. There was a significant difference (P <0.05) between the Xuezhikang intervention group and high-fat control group (P <0.05), and hyperlipemia metabolomics related markers, oxalic acid, butyric acid, mannitol, glucose, glucuronic acid were found. Glucuronic acid and non-binding bilirubin combined with bilirubin, combined with some of the liver harmful substances, play a detoxification effect.

Conclusion: The results of metabonomics showed that the high-fat group and the control group have significant differences. Mannose, glucose content is relatively stable, lipid metabolism in high-fat group stearic acid, palmitic acid levels decreased, suggesting that high-fat diet disorders rat body lipid metabolism. It is worth mentioning that the experimental evaluation of rats, such as biochemical indicators and pathological results are prompted to model success, Xuezhikang intervention effect is more significant, consistent with the expected.

Keywords: Xuezhikang, hyperlipidemia, metabolomics, GC-MS, detoxification, glucuronic acid.

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Article Details

VOLUME: 17
ISSUE: 6
Year: 2021
Page: [702 - 709]
Pages: 8
DOI: 10.2174/1573412916999200416132311
Price: $95

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