Title:Differences in the Expression Pattern of mRNA Protein SEMA3F in Endometrial Cancer <i>in vitro</i> under Cisplatin Treatment
VOLUME: 21 ISSUE: 11
Author(s):Przemysław Kieszkowski*, Dariusz Dąbruś, Beniamin O. Grabarek and Dariusz Boroń
Affiliation:Voivodeship Specialist Hospital in Wloclawek, Wloclawek, Faculty of Health Science, Public Higher Medical Professional School in Opole, Opole, Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology in Katowice, Zabrze, Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology in Katowice, Zabrze
Keywords:SEMA3F, endometrial cancer cell line, cisplatin, expression, supplementary molecular marker, gene transcript.
Abstract:
Background: Semaphorin 3F (SEMA3F) plays a substantial role in carcinogenesis, because
of its role in inducing angiogenesis, and creating a microenvironment for the developing tumor.
Objective: The purpose of this work was to assess the impact of cisplatin, depending on the concentration
and exposure time on the expression pattern of SEMA3F in an endometrial cancer cell line.
Materials and Methods: Cultures of the Ishikawa endometrial cancer cells were incubated with cisplatin
with the following concentrations: 2.5μM; 5μM; and 10μM and for the following periods of time:
12; 24; and 48 hours. Cells not incubated with the drug constituted the control in the experiment. To
determine the effect of cisplatin on the expression of SEMA3F, the real-time quantitative reverse transcription
reaction (RtqPCR; mRNA) was used, as well as the ELISA assay (protein). The statistical
analysis was done with the admission of p<0.05.
Results: The silencing of SEMA3F expression on the transcriptome and proteome levels in a culture
unexposed to the effects of cisplatin in comparison to endometrial cancer cells under the influence of
cisplatin (p<0.05) were noted. Along with an increase in the concentration of the drug used, the number
of copies of the gene transcript, during the shortest incubation period had a gradual increase. Only
for the highest concentration of the drug, substantial statistical differences in the expression of the
SEMA3F protein between 24 and 48 hour incubation periods (p<0.05) were determined.
Conclusion: Using cisplatin in an endometrial cancer cell culture results in an increased expression of
SEMA3F, which advantageously affects the normalization of the neoplastic angiogenic process and
lowers the proliferation of the cells making up the mass of the tumor.
