Simultaneous Analysis of Sexual Stimulants and Anabolic Steroids as Adulterants in Dietary Supplements by High Performance Liquid Chromatography with Photodiode Array Detection

Author(s): Carine Viana*, Gabriela Zanella Marcon, Thais Ramos Dal Molin, Denise Bohrer, Paulo Cícero do Nascimento, Leandro M. de Carvalho

Journal Name: Current Pharmaceutical Analysis

Volume 17 , Issue 6 , 2021


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Abstract:

Background: The illegal virtual market for food supplements facilitates fraud and adulteration. Worldwide concern for consumer safety is growing on the part regulatory agencies, healthcare professionals and consumers.

Objective: This work aimed to evaluate the presence of sexual stimulants and anabolic steroids commonly used in the adulteration of dietary supplements through the development of a high performance liquid chromatography with the photodiode array detection (HPLC-DAD) method for the identification and quantification of these compounds.

Methods: The mobile phase composed of an ammonium acetate solution, acetonitrile and methanol led to the efficient separation of vardenafil, testosterone base, testosterone propionate, tadalafil, sildenafil and yohimbine.

Results: The assay was linear (r2> 0.999), precise (RSD% <0.5), accurate (99.1 to 105.2%), and the limits of detection and quantification were less than 0.05 and 0.15 μg/mL, respectively. Four samples of dietary supplements contained testosterone (n=1), tadalafil (n=2) and yohimbine (n=1) as adulterants. The adulterants found were in subtherapeutic doses, probably to reduce possible adverse effects and the action expected to appear natural. Since about 80% of adverse drug reactions are dose dependent, unpredictable adverse drug reactions are dose independent and based on idiosyncratic or allergic mechanisms or intolerance.

Conclusion: The developed method is convenient and easily applicable for adulteration detection of the analyzed drugs in the multicomponent supplements.

Keywords: Adulterants, anabolic steroids, dietary supplements, liquid chromatography with photodiode array, sexual stimulants.

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Article Details

VOLUME: 17
ISSUE: 6
Year: 2021
Page: [767 - 773]
Pages: 7
DOI: 10.2174/1573412916999200415175325
Price: $95

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