Genetic Diversity and Drug Resistance of HIV-1 CRF55_01B in Guangdong, China

Author(s): Guolong Yu*, Yan Li, Xuhe Huang, Pingping Zhou, Jin Yan, Xinge Yan, Xiaobing Fu, Yang Fang, Jun Liu, Jie Li, Xiang He, Peng Lin

Journal Name: Current HIV Research

Volume 18 , Issue 3 , 2020


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Background: HIV-1 CRF55_01B was first reported in 2013. At present, no report is available regarding this new clade’s polymorphisms in its functionally critical regions protease and reverse transcriptase.

Objective: To identify the diversity difference in protease and reverse transcriptase between CRF55_01B and its parental clades CRF01_AE and subtype B; and to investigate CRF55_01B’s drug resistance mutations associated with the protease inhibition and reverse transcriptase inhibition.

Methods: HIV-1 RNA was extracted from plasma derived from a MSM population. The reverse transcription and nested PCR amplification were performed following our in-house PCR procedure. Genotyping and drug resistant-associated mutations and polymorphisms were identified based on polygenetic analyses and the usage of the HIV Drug Resistance Database, respectively.

Results: A total of 9.24 % of the identified CRF55_01B sequences bear the primary drug resistance. CRF55_01B contains polymorphisms I13I/V, G16E and E35D that differ from those in CRF01_AE. Among the 11 polymorphisms in the RT region, seven were statistically different from CRF01_AE’s. Another three polymorphisms, R211K (98.3%), F214L (98.3%), and V245A/E (98.3 %.), were identified in the RT region and they all were statistically different with that of the subtype B. The V179E/D mutation, responsible for 100% potential low-level drug resistance, was found in all CRF55_01B sequences. Lastly, the phylogenetic analyses demonstrated 18 distinct clusters that account for 35% of the samples.

Conclusion: CRF55_01B’s pol has different genetic diversity comparing to its counterpart in CRF55_01B’s parental clades. CRF55_01B has a high primary drug resistance presence and the V179E/D mutation may confer more vulnerability to drug resistance.

Keywords: CRF55_01B, genetic diversity, drug resistance mutations, MSM, CRF01_AE, B subtype.

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Article Details

VOLUME: 18
ISSUE: 3
Year: 2020
Published on: 11 June, 2020
Page: [210 - 218]
Pages: 9
DOI: 10.2174/1570162X18666200415140652

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