Aim and Objective: Humans continuously use pesticides in the field to control the pest
population and weeds for considerable agricultural productivity. Side-by species like grazinganimals,
insects and other species are adversely affected by or become resistant to pesticides. Insects,
birds and cattle are highly abundant dwellers of the agriculture-field and represent three distinct phyla
having versatile physiological features. Besides higher agricultural-productivity, protection to several
species will maintain ecological/environmental balance. Studies on the effect of widely used
pesticides on their DNA-stability and important enzymatic-activities are insufficient.
Materials and Methods: Antioxidant-activity (Superoxide-dismutase; SOD/Catalase- by gelzymogram-
assay) and DNA-stability (fragmentation-assay) in hepatic/gut tissues were studied
after in vitro exposure of Chlorpyrifos, Fenvalerate, Nimbecidine or Azadirachtin to
Results: In general, all pesticides were found to impair enzymatic-activities. However, lower
organisms were affected more than higher vertebrates by azadirachtin-treatment. DNA
fragmentation was found more in insects/poultry-birds than that of the cattle in hepatic/gut tissues.
Inversely, toxicity/antioxidant marker-enzymes were more responsive in insect gut-tissues.
However, mitochondrialtoxicity revealed variable effects on different species. It has been noticed
that chlorpyrifos is the most toxic pesticide, followed by Fenvalerate/Nimbecidine (Azadirachtin,
AZT). Nevertheless, AZT revealed its higher DNA-destabilizing effects on the field-insects as
compared to the other animals.
Conclusion: Field-insects are highly integrated into the ecosystem and the local bio-geo-chemical
cycle, which may be impaired. Pesticides may have toxic effects on higher vertebrates and may
sustain in the soil after being metabolized into their different derivatives. Some of the sensitive
biochemical parameters of this organism may be used as a biomarker for pesticide toxicity.