Objective: A particular, easy, and precise reverse phase liquid chromatographic technique
was developed for the determination of Zaltoprofen in human plasma.
Methods: The ODS C18 (250mm x 4.6mm, 5μm) column was utilized for determination. The mobile
phase contains buffer and acetonitrile (55:45 v/v). The rate of flow was 1.0 ml/min. The volume of infused
sample was 10 μl. The column was kept at a temperature of ~30°C and equilibrated for no less
than 30 min, prior to injection of the solutions. The detection wavelength was set at 331 nm.
Results: The linearity experiment was performed for Zaltoprofen in the range of 0.15 to 20 μg/ml. The
observed recovery of Zaltoprofen was 98.32 %.
Conclusion: The suggested technique has been validated and has proved to be very helpful for zaltoprofen
determination in human plasma.