Bio-Analytical Method Development and Validation for Estimation of Zaltoprofen in Human Plasma by Reverse Phase -HPLC Method

Author(s): Kishor Kumar Erukulla, S.R. Suseem*

Journal Name: Current Pharmaceutical Analysis

Volume 17 , Issue 6 , 2021


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Abstract:

Objective: A particular, easy, and precise reverse phase liquid chromatographic technique was developed for the determination of Zaltoprofen in human plasma.

Methods: The ODS C18 (250mm x 4.6mm, 5μm) column was utilized for determination. The mobile phase contains buffer and acetonitrile (55:45 v/v). The rate of flow was 1.0 ml/min. The volume of infused sample was 10 μl. The column was kept at a temperature of ~30°C and equilibrated for no less than 30 min, prior to injection of the solutions. The detection wavelength was set at 331 nm.

Results: The linearity experiment was performed for Zaltoprofen in the range of 0.15 to 20 μg/ml. The observed recovery of Zaltoprofen was 98.32 %.

Conclusion: The suggested technique has been validated and has proved to be very helpful for zaltoprofen determination in human plasma.

Keywords: Zaltoprofen, validation, method, buffer, human plasma, NSAID.

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Article Details

VOLUME: 17
ISSUE: 6
Year: 2021
Page: [774 - 781]
Pages: 8
DOI: 10.2174/1573412916999200409105530
Price: $95

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