Background: Curcuma longa Linn. and Cratoxylum formosum have
been consumed as the Thai traditional medicine for curing liver diseases. However,
biological effects from a combination of C. longa and C. formosum have not
Objective: This study focused to investigate the effects of combined extracts on
anti-liver cancer activity, anti-Hepatitis B virus (HBV) activity and alteration of
miRNA expression, including their effects on p53 and NF-κB protein expressions.
Methods: The cytotoxicity effects of hot water extract on liver cancer cells were
examined by using the MTT assay. Annexin V assay and Western blot analysis
were conducted to investigate the effects of plant extracts on the apoptosis pathway
and cellular protein expressions. Finally, the expression of HBV genes and
miRNA expressions were evaluated by using a quantitative real-time PCR.
Results: The cell viability assay indicated that 400 μg/mL of combined C. longa
and C. formosum extracts significantly inhibited different liver cancer cell lines
without affecting a normal cell (Vero). The combined extracts could induce liver
cancer cell death via the apoptosis pathway by up-regulating p53 expression
while down-regulating NF-κB expression. Moreover, 150 μg/mL of the combined
extract specifically suppressed HBx gene expression in liver cancer cells
that stably express HBV proteins. However, 150 μg/mL of the combined extract
had no effect on miR-34a and miR-199a/b expressions.
Conclusion: This study firstly reported anti-liver cancer and anti-HBV activities
of the combined C. longa and C. formosum extracts.