Background: Significant advances have been occurred in analytical research since
the 1970s by liquid chromatography (LC) as the separation method. Reverse phase liquid
chromatography (RPLC) method, using hydrophobic stationary phases and polar mobile
phases is the most commonly used chromatographic method. However, it is difficult to
analyze some polar compounds with this method. Another separation method, normal phase
liquid chromatography (NPLC), which involves polar stationary phases with organic eluents.
NPLC presents low-efficiency separations and asymmetric chromatographic peak shapes
when analyzing polar compounds. Hydrophilic interaction liquid chromatography (HILIC) is
an interesting and promising alternative method for the analysis of polar compounds. HILIC
is be defined a separation method which combines stationary phases used in the NPLC
method and mobile phases used in RPLC method. HILIC can be successfully applied to all
types of liquid chromatographic separations such as pharmaceutical compounds, small
molecules, metabolites, drugs of abuse, carbohydrates, toxins, oligosaccharides, peptides,
amino acids and proteins.
Objective: This paper provides a general overview of the recent application of HILIC in the
pharmaceutical research in the different sample matrices such as pharmaceutical dosage form,
plasma, serum, environmental samples, animal origin samples, plant origin samples, etc. Also,
this review focuses on the most recent and selected papers in the drug research from 2009 to
submission date in 2020 dealing with the analysis of different components using HILIC.
Results and Conclusion: The literature survey showed that HILIC applications are increasing
every year in pharmaceutical research. It was found that HILIC allows simultaneous analysis
of many compounds using different detectors.