Background: Propolis is a resinous mixture, collected by honeybees from tree bud and exudates of the plants.
Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are
responsible for its antitumor, anticancer, antiviral and antifungal effects. Propolis should be extracted and the
solubility of propolis differs depending on the solvent used. Solvents used for propolis extraction should solve
the propolis and be nontoxic.
Objective: In this study, raw propolis was extracted by peppermint and clove volatile oil.
Methods: Chemical composition of extracts was determined by using GC-MS equipment. Total phenolic content
and antioxidant activity of the extracts was measured. α-amylase inhibitory activity of the extracts was carried
out as well.
Results: The findings of the present study showed that clove volatile oil is more effective on extraction of
propolis than peppermint volatile oil. Total phenolic content of these extracts was determined as 175.12 and
40.80 mg GAE/mL for clove and peppermint oil propolis extract respectively. All extracts contained the same
phenolic compounds but less quantity in volatile oil extract than ethanol extract. Both of these extracts showed
better α-amylase ınhibitory activity than a reference inhibitor, acarbose.
Conclusion: It could be concluded that propolis extract obtained by using volatile oils could be used as
complementary agent in the treatment of type 2 diabetes mellitus.