Background: Osteosarcoma is one of the most serious primary malignant bone tumors
that threaten the lives of children and adolescents. However, the mechanism underlying and how to
prevent or treat the disease have not been well understood.
Aims and Objective: This aim of the present study was to identify the key genes and explore novel
insights into the molecular mechanism of miR-542-3p over-expressed Osteosarcoma.
Materials and Methods: Gene expression profile data GDS5367 was downloaded from the Gene
Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were screened
using GEO2R, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG)
analyses were performed using the DAVID database. And protein-protein interaction (PPI)
network was constructed by the STRING database. In addition, the most highly connected module
was screened by plugin MCODE and hub genes by plugin CytoHubba. Furthermore, UALCAN
and The Cancer Genome Atlas were performed for survival analysis.
Result: In total, 1421 DEGs were identified, including 598 genes were up-regulated and 823 genes
were down-regulated. GO analysis showed that DEGs were classified into three groups and DEGs
mainly enriched in Steroid biosynthesis, Ubiquitin mediated proteolysis and p53 signaling
pathway. Six hub genes (UBA52, RNF114, UBE2H, TRIP12, HNRNPC, and PTBP1) may be key
genes with the progression of osteosarcoma.
Conclusion: The results could better understand the mechanism of osteosarcoma, which may
facilitate a novel insight into treatment targets.