Background: Diosmetin (DIOS) is the aglycone of the flavonoid glycoside, diosmin, derived
naturally from the leaves of the legume, Olea europaea, and Acacia farnesiana. It has potent
anticancer activity against multiple forms of cancers. However, the role of DIOS in renal carcinoma
and its mechanism of action remain unclear.
Objective: The purpose of this study is to investigate the effect of DIOS on cell viability and apoptosis
in renal carcinoma cells and explore the possible mechanism of action.
Methods: Cell viability, cytotoxicity, caspase activity, apoptosis, and expression of apoptotic related
proteins were analyzed in renal carcinoma ACHN cells.
Results: The results showed that DIOS inhibited the cell viability, and induced cytotoxicity and
apoptosis in ACHN cells. Furthermore, DIOS increased expression of p53 mRNA and proteins,
and downregulated phosphorylation of the phosphoinositide 3-kinase and protein B kinase
(PI3K/AKT). In addition, it was observed that the anticancer effect of DIOS was significantly enhanced
by the p53 activator, but inhibited by the p53 inhibitor.
Conclusion: Our data suggested that DIOS induced apoptosis in renal carcinoma ACHN cells by
reducing AKT phosphorylation through p53 upregulation.