MiR-493 Induces Cytotoxic Autophagy in Prostate Cancer Cells through Regulation on PHLPP2

(E-pub Ahead of Print)

Author(s): Jun Deng, Ming Ma, Wei Jiang, Liangliang Zheng, Suping Cui*

Journal Name: Current Pharmaceutical Biotechnology

Become EABM
Become Reviewer

Abstract:

Aims: We aimed to explore the relationship between miR-493 and autophagy in PC.

Background: MiR-493 promotes the proliferation of prostate cancer (PC) cells by targeting PHLPP2.

Objective: To explore the relationship between miR-493 and autophagy in PC.

Method: QRT-PCR and western blotting were used to determine the mRNA levels and protein expression of miR-493, PHLPP2, autophagy gene BECN1 and ATG7 in PC cells. The autophagy gene expression was determined after PC cells transfected with miR-493 precursor or PHLPP2 precursor. Corresponding changes of autophagy phenotype and PC cell function were also studied.

Result: The mRNA levels and protein expression of miR-493, PHLPP2, BECN1 and ATG7 in PC cells were significantly increased. Overexpression of miR-493 or PHLPP2 markedly upregulated the expression levels of BECN1 and ATG7 in PC cells. Overexpression of miR-493 and PHLPP2 markedly promoted autophagy, and inhibited the invasion and cloning formation of PC cells.

Conclusion: MiR-493 is a potent inducer of cytotoxic autophagy that leads to prostate cancer inhibition by regulating on PHLPP2.

Keywords: MiR-493, Prostate cancer, PHLPP2, Autophagy , QRT-PCR, PC cells, Overexpression

Rights & PermissionsPrintExport Cite as

Article Details

(E-pub Ahead of Print)
DOI: 10.2174/1389201021666200318120733
Price: $95

Article Metrics

PDF: 2