MiR-493 Induces Cytotoxic Autophagy in Prostate Cancer Cells through Regulation on PHLPP2

Author(s): Jun Deng, Ming Ma, Wei Jiang, Liangliang Zheng, Suping Cui*

Journal Name: Current Pharmaceutical Biotechnology

Volume 21 , Issue 14 , 2020


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Abstract:

Background: MiR-493 promotes the proliferation of prostate cancer (PC) cells by targeting PHLPP2. We aimed to explore the relationship between miR-493 and autophagy in PC.

Methods: qRT-PCR and western blotting were used to determine the mRNA levels and protein expression of miR-493, PHLPP2, autophagy gene BECN1 and ATG7 in PC cells. The autophagy gene expression was determined after PC cells transfected with miR-493 precursor or PHLPP2 precursor. Corresponding changes of autophagy phenotype and PC cell function were also studied.

Results: The mRNA levels and protein expression of miR-493, PHLPP2, BECN1 and ATG7 in PC cells were significantly decreased in PC cells. Overexpression of miR-493 or PHLPP2 markedly upregulated the expression levels of BECN1 and ATG7 in PC cells. Overexpression of miR-493 and PHLPP2 markedly promoted autophagy, and inhibited the invasion and cloning formation of PC cells.

Conclusion: MiR-493 is a potent inducer of cytotoxic autophagy that leads to prostate cancer inhibition by regulating on PHLPP2.

Keywords: MiR-493, Prostate cancer, PHLPP2, autophagy, qRT-PCR, PC cells.

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Article Details

VOLUME: 21
ISSUE: 14
Year: 2020
Page: [1451 - 1456]
Pages: 6
DOI: 10.2174/1389201021666200318120733
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