Introduction: Nowadays, the co-infection of different classes of pathogens is a major
concern. The objective of this study was to develop a successful therapy for keratitis caused by the
co-infection of Candida sp. with Pseudomonas sp, which is difficult to cure. The study is based on
a 47 years old male farmer showing redness and watering in the right eye for 15-days.
Methods: The microbiological examination was performed to isolate the causative organisms, i.e.
Pseudomonas aeruginosa and Candida albicans. They were cultured separately along with their
co-culture and treated with ciprofloxacin and amphotericin B during the growing stage to predict a
Results: Scanning electron microscope (SEM) results confirmed the inter-specific interaction between
the two different types of microorganisms. Amphotericin-B and Ciprofloxacin showed the
least MIC value for both organisms in co-culture.
Conclusion: Treatment with Amphotericin-B and 5% ciprofloxacin effectively hindered the
growth of Pseudomonas aeruginosa and Candida albicans, the co-infection of which caused keratitis.
This therapy may be successfully implied for such cases of co-infection in the future.