Background: Clerodendrum paniculatum has ethnomedicinal importance in treatment of
disorders like wound, typhoid, jaundice, malaria and anemia.
Objective: To evaluate the antioxidant and hepatoprotective activity of Clerodendrum paniculatum
leaves against carbon tetrachloride (CCl4) induced rat model and identification of its bioactive constituents
by Gas Chromatography Mass Spectroscopy (GC MS).
Methods: Successive solvent extraction was carried out. Total phenolic, flavonoid content and antioxidant
activity by 2,2- diphenyl-1-picryl hydrazyl (DPPH), nitric oxide and 2-Azino-bis [3-ethyl benzothiazoline-
6-sufonic acid] (ABTS method) were done. Ethyl acetate extract was selected for hepatoprotective
study in carbon tetrachloride intoxicated model followed by the measurement of liver function
marker enzymes such as SGOT (Serum Glutamate Oxaloacetate Transaminase), SGPT (Serum
Glutamate Pyruvate Transaminase), and ALP (Alkaline Phosphatase). Biochemical parameters like
bilirubin and protein were measured. Histopathologic liver sections were carried out. Bioactive constituents
were evaluated by GC MS.
Results: By DPPH and ABTS method, ethyl acetate extract showed IC50 as 70.14±0.92
μg/ml,2958.24±2.460 μg/ml, respectively. The alcoholic extract showed maximum IC50 (197.22 ±7.16
μg/ml) by Nitric oxide radical scavenging method. Hepatoprotective study reveals that intoxicated
animal groups have elevated levels of enzymes and bilirubin and suppress the production of protein.
The extract pre-treatment showed a significant decrease in enzymes and increased production of total
protein in a dose-dependent manner. Histopathologic studies also support the hepatoprotective activity.
GC MS analysis revealed the presence of seven major bioactive constituents with ethyl palmitate as the
Conclusion: The results support the proof for the hepatoprotective potential of the CPLE extract with
potent antioxidant activity and enhanced liver enzyme level. The observed activity could be due to the
presence of bioactive compounds as identified by GC MS analysis.