In Silico and In Vitro Studies of Natural Compounds as Human CK2 Inhibitors

Author(s): Samer Haidar*, Franziska Jürgens, Dagmar Aichele, Joachim Jose

Journal Name: Current Computer-Aided Drug Design

Volume 17 , Issue 2 , 2021


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Abstract:

Background: Casein Kinase 2 (CK2) is a ubiquitous cellular serine-threonine kinase with broad spectrum of substrates. This enzyme is widely expressed in eukaryotic cells and is overexpressed in different human cancers. Thus, the inhibition of CK2 can induce the physiological process of apoptosis leading to tumor cell death.

Objectives: Selecting natural inhibitors toward the target enzyme using database mining.

Methods: With our continuous effort to discover new compounds with CK2 inhibitory effect, several commercial natural databases were searched using molecular modeling approach and the selected compounds were evaluated in vitro.

Results: Three compounds were selected as candidates and evaluated in vitro using CK2 holoenzyme, their effect on three cancer cell lines was determined. The selected candidates were weak inhibitors toward the target enzyme, only one compound showed moderate effect on cell viability.

Conclusion: Several natural databases were screened, compounds were selected and tested in vitro. Despite the unexpected low inhibitory activity of the tested compounds, this study can help in directing the search of potent CK2 inhibitors and better understand the binding requirements of the ATP competitive inhibitors.

Keywords: In silico screening, CK2, natural compounds, inhibitors, in vitro, cancer.

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Article Details

VOLUME: 17
ISSUE: 2
Year: 2021
Published on: 18 May, 2021
Page: [323 - 331]
Pages: 9
DOI: 10.2174/1573409916666200311150744
Price: $65

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