Background: Targeted drug delivery is one of the recent hot topics in cancer therapy. Because of
having a targeting potential under the magnetic field and a suitable surface for the attachment of different therapeutic
moieties, magnetic nanoparticles are widely studied for their applications in medicine.
Objective: Gemcitabine loaded polyhydroxybutyrate coated magnetic nanoparticles (Gem-PHB-MNPs) were
synthesized and characterized for the treatment of breast cancer by the targeted drug delivery method.
Methods: The characterization of nanoparticles was confirmed by FTIR, XPS, TEM, and spectrophotometric
analyses. The cytotoxicities of drug-free nanoparticles and Gemcitabine loaded nanoparticles were determined
with cell proliferation assay using SKBR-3 and MCF-7 breast cancer cell lines.
Results: The release of Gemcitabine from PHB-MNPs indicated a pH-dependent pattern, which is a desirable
release characteristic, since the pH of the tumor microenvironment and endosomal structures are acidic, while
bloodstream and healthy-tissues are neutral. Drug-free PHB-MNPs were not cytotoxic to the SKBR-3 and MCF-
7 cells, whereas the Gemcitabine loaded PHB-MNPs was about two-fold as cytotoxic with respect to free Gemcitabine.
In vitro targeting ability of PHB-MNPs was shown under the magnetic field.
Conclusion: Considering these facts, we may suggest that these nanoparticles can be a promising candidate for
the development of a novel targeted drug delivery system for breast cancer.