Title:Toxicity Evaluation of the Subacute Diazinon in Aged Male Rats: Hematological Aspects
VOLUME: 20 ISSUE: 3
Author(s):Saeed Samarghandian, Tahereh Farkhondeh and Shahnaz Yousefizadeh*
Affiliation:Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Medical Toxicology and Drug Abuse Research Center (MTDRC), Birjand University of Medical Sciences (BUMS), Birjand, Department of Laboratory and Clinical Sciences, Faculty of Para-Veterinary, Ilam University, Ilam
Keywords:Aging, diazinon, hematology, organophosphate, rat, diabetes.
Abstract:
Background & Objective: Age-dependent Organophosphates (OPs) toxicity is a controversial
topic. The present study was designed to investigate the effect of the sub-acute exposure to
diazinon (DZN), one of the main OPs insecticides, on the hematological alterations in adult and
aged male rats.
Methods: For the aim of this approach, the adult and aged rats were administered with DZN (15 mg/kg,
orally) for 4 weeks. Then, the blood samples were collected from the retro-orbital sinus for measuring
red blood cell (RBC), hemoglobin (Hb), hematocrit (Hct), platelets (PLT), MCV (mean corpuscular
volume), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin (MCHC).
Results: The obtained results indicated that DZN significantly decreased RBCs (4.93 ± 0.41), Htc
(28.12 ± 1.21), Hb (10.31 ± 0.36), MCHC (30.51 ± 2.04), MCV (62.86 ± 2.58), and PLT (265.6 ±
34.81) values in the adult and aged rats versus the age-matched control rats. Moreover, RBC, Hb,
and Htc levels decreased significantly in the aged rats versus adult rats. However, no significant
differences were observed between MCHC, MCV, and PLT levels in adult and aged rats. Moreover,
the MCH concentration did not change in any group. Additionally, DZN did not deteriorate the hematological
alterations in the aged rats versus adult rats.
Conclusion: the present study showed that the toxicity of DZN is not associated with age. However,
more studies should be conducted to confirm this finding.
