Objective: The aim of the present study is to isolate and characterize the bioactive compounds
from Pleurotus djamor against human breast cancer (MDA-MD-231) and mouse T cell lymphoma
(EL4) cell lines.
Materials and Methods: Sequential fractionization and column chromatography methods were involved
in compound isolation. The structures of the isolated compound were determined by NMR,
GC/MS, and X-ray crystallography studies.
Results: The isolated compounds 1- 4 [D-mannitol (C1), ergosta-5,7,22-trien-3β-ol (C2), 5,8-
epidioxy-ergosta-6-22-dien-3β-ol (C3), and palmitic acid (C4)] are white crystal and amorphous
powder in nature. All these compounds were isolated from this mushroom for the first time. In vitro
lipid peroxidation activities of isolated compounds were determined by ferric thiocyanate (FTC) and
thiobarbituric acid (TBA) method. The sterol derivatives C2 and C3 compounds displayed strong antioxidant
activity and were not significantly different (p<0.05) to α-tocopherol. This finding elaborates
on the isolation of a cytotoxic compound C2 and C3 from P. djamor via a rapid elution method.
Conclusion: The compound C3 has exhibited better cytotoxic activity against MDA-MD-231 and
EL4 cells. The present finding and data might provide new insights into the possible therapeutic and
pharmaceutical use for the design of anti-cancer drugs from this edible mushroom.