Standardization Using Analytical Techniques (UV, NMR, FTIR, HPLC, Mass) and Pharmacognostic Evaluation of the Roots of Selinum vaginatum: A Rare Himalayan Plant of the Rohtang Region

Author(s): Nikita Saraswat*, Neetu Sachan, Phool Chandra

Journal Name: Current Biotechnology

Volume 9 , Issue 2 , 2020

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Graphical Abstract:


Introduction: Selinum vaginatum is an endemic Indian plant present on high altitude and is known as “Bhootkeshi,” by the locals. The root of plant Selinum vaginatum commonly known as “Bhootkeshi” from the family Umbellifercae is an aromatic herbal drug. Selinum vaginatum has been used traditionally for curing seizures, epilepsy, mental illness, and hysteria. This plant has shown therapeutic effects as used by the locals. There is no data available regarding standardization of the roots of Selinum vaginatum. It is a novel work as the plant is rarely found and shows medicinal values but no quality control work has been performed.

Objective: The aim of this paper is to study quality control and carry out physicochemical analysis and standardization of the roots of Selinum vaginatum for determining its quality and purity. As no work has been performed on the roots of this plant, so standardization will help in keeping a record of the parameters exhibited during the evaluation of the roots of Selinum vaginatum.

Methods: The organoleptic characteristics were studied and physicochemical tests, phytochemical analysis, fluorescence analysis, TLC, UV, FTIR, NMR, and Electrospray Ionisation - Mass spectroscopy, HPLC and other parameters were carried out for the standardization of the test drug. The procedures were carried out as per the WHO standards. The total ash values, swelling index, foaming index, moisture content, alcohol, and water-soluble extractive values were calculated. The TLC profile of Selinum vaginatum was observed and the UV, FTIR, HPLC, NMR and Mass spectroscopies of the plant were also performed.

Results: The microscopic results showed the presence of schizogenous canals in abundance, cork cells, oil and globules, while medullary rays and pith were absent and around 10-17 layers of cells were seen. The presence of active compounds like phenols, terpenoids, alkaloids, coumarins, essential oils, tannins, saponins, carbohydrates, and acids was reported by phytochemical tests, TLC, UV, NMR, HPLC, Mass, and FTIR spectroscopy methods. These compounds can be studied in detail for understanding the potential pharmacological benefits and decoding the traditional uses. The spectroscopic analysis and physicochemical tests have shown the presence of phenols, esters, organic halogens, carbohydrate, carboxylic acids, alcohols, ethers, and glycosides which must be responsible for the medicinal properties of the plant.

Conclusion: This study has provided a set of information that will be useful for the identification of fresh batches of the roots of Selinum vaginatum and this can be used as a reference set of information for accessing the purity, quality and identification, and analysis of the authenticity of the plant roots. Spectroscopic techniques have shown peaks, which on interpretation have reported the presence of active compounds - (coumarins like selenidin, angelicin, vaginidine, furocoumarin - oroselol and coumarin derivative- lomatin, isovaleric acid, Valeranone, Tricyclene, p-Cymene, α-Pinene, β-Pinene, Camphene, Borneol, p- Camphene, cholorgenic acid, β-Myrcene, Limonene and α-Pineneoxide which can be studied for understanding and identifying the pharmacological actions of the rhizomatous roots of S. vaginatum. All the information collected by research on the roots of S. vaginatum will help in the identification of crude drugs and also in the development of a standard for the drug Selinum vaginatum.

Keywords: Spectroscopy, quality control, phyto-chemical analysis, ethno-medicine, indigenous medicine, himalayan medicine.

Nikam PH, Kareparamban J, Jadhav A, Kadam V. Future trends in standardization of herbal drugs. J Appl Pharm Sci 2012; 2(6): 38-44.
The Ayurvedic Pharmacopoeia of India. Ministry of health and family welfare, Department of AYUSH 2001; Part-1 4: 12-4.
Anupama. Bhutakeshi information, medicinal uses and more 2017. Available from:
Chauhan NS. Medicinal and aromatic plants of Himachal Pradesh. New Delhi, India: Indus 1999.
Seshadri TR, Sood MS, Handa KL. Chemical components of the roots of Selinum vaginatum—1: Coumarins of the petroleum ether extract. Tetrahedron 1967; 23(4): 1883-91.
Tewari M, Mathela CS. Chemical composition of the leaf and root oils of Selinum tenuifolium. Indian Perfumer 2003; 47: 343-5.
Rodilla JM, Tinoco MT, Morais JC, et al. Laurus novocanariensis essential oil: Seasonal variation and valorization. Biochem Syst Ecol 2008; 36(3): 167-76.
Choi WS, Park BS, Lee YH, Jang DY, Yoon HY, Lee SE. Fumigant toxicities of essential oils and monoterpenes against Lycoriella mali adults. Crop Prot 2006; 25(4): 398-401.
Saraswat N, Sachan N, Chandra P. A detailed review on the rarely found Himalayan Herb Selinum vaginatum: Its active constituents, pharmacological uses, traditional and potential benefits. Pharmacophore 2020; 11(2): 40-52.
Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 1996; 20(7): 933-56.
[ PMID: 8743980]
Mattila P, Hellström J. Phenolic acids in potatoes, vegetables, and some of their products. J Food Compos Anal 2007; 20(3-4): 152-60.
Devkota H P, Adhikari B, Watanabe T, Yahara S. Nonvolatile chemical constituents from the leaves of Ligusticopsis wallichiana (DC.) Pimenov Kljuykov and their free radical-scavenging activity J An Methods Chem 2018.
Evans WC. Trease and Evans Pharmacognosy. 15th ed. London: WB Sounders Company Ltd, UK 2005; p. 5457.
Sass JE. Elements of botanical micro technique. New York, USA: Mc Graw Hill Book Co 1940; p. 222.
Johanson DA. Plant Micro Technique. New York, USA: Mc Graw Hill Book Co 1940.
Sofowora A. Medicinal plants and traditional medicine in Africa. 3rd ed. Ibadan: Spectrum Books Limited 2008.
WHO-Quality Control methods for medicinal plants material. World Health Organization, Geneva, AIT US publisher and distributor Delhi 1990.
Pandey MM, Katara A, Pandey G, Rastogi S, Rawat AKS. An important Indian traditional drug of Ayurveda jatamansi and its substitute Bhootkeshi: Chemical profiling and antimicrobial activity. Evi. Based Com. Alter. Med 2013.
Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. PID New Delhi 1990; 1: 369.
Khandelwal KR. Practical Pharmacognosy.. Pune: Nirali prakashan 2004; 149-60.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 39th ed. Pune Nirali Prakashan 2007; pp. 108-9.
Lachman L, Lieberman HA, Kanig JL. The theory and practice of industrial pharmacy. 3rd ed. Mumbai: Verghese Publishing House, India 1987; pp. 183-316.
Aulton ME. Pharmaceutics, the science of dosage forms designs. 2nd ed. New Delhi, India: Churchill Livingstone 2002; pp. 205-21.
Chatwal GR, Anand SK. Instrumental methods of chemical analysis. Mumbai: Himalaya Publishing House 2002; p. 317.
Chandra P, Kishore K, Ghosh AK. Assessment of antisecretory, gastroprotective, and in-vitro antacid potential of Daucus carota in experimental rats. Osong Public Health Res Perspect 2015; 6(6): 329-35.
[] [PMID: 26835241]
Chandra P, Kishore K, Ghosh AK. Evaluation of antisecretory, gastroprotective and in-vitro antacid capacity of Fumaria indica in rats. J Environ Biol 2015; 36(5): 1137-42.
[PMID: 26521557]
Chandra P, Sachan N, Kishore K, Ghosh AK. Acute, sub-chronic oral toxicity studies and evaluation of antiulcer activity of Sooktyn in experimental animals. J Adv Pharm Technol Res 2012; 3(2): 117-23.
[] [PMID: 22837960]
Chandra P, Sachan N, Pal D. Protective effect of Dalbergia sissoo Roxb. ex DC. (family: Fabaceae) leaves against experimentally induced diarrhoea and peristalsis in mice. Toxicol Ind Health 2015; 31(12): 1229-35.
[] [PMID: 23788395]
Chandra P, Yadav E, Mani M, Ghosh AK, Sachan N. Protective effect of Lygodium flexuosum (family: Lygodiaceae) against excision, incision and dead space wounds models in experimental rats. Toxicol Ind Health 2015; 31(3): 274-80.
[] [PMID: 23299194]
Pal D, Mishra P, Sachan N, Ghosh AK. Biological activities and medicinal properties of Cajanus cajan (L) Millsp. J Adv Pharm Technol Res 2011; 2(4): 207-14.
[] [PMID: 22247887]
Sachan N, Chandra P, Pal D. Effect of Delonix regia (Boj. Ex Hook.) Raf. stem bark extract against experimentally induced ulcers in rats. Indian J Exp Biol 2017; 55(1): 49-54.
[PMID: 30183229]
Yadav E, Mani M, Chandra P, Sachan N, Ghosh AK. A review on therapeutic potential of Lygodium flexuosum Linn. Pharmacogn Rev 2012; 6(12): 107-14.
[] [PMID: 23055636]
Saraswat N, Sachan N, Chandra P. A review on ethnobotanical, phytochemical, pharmacological and traditional aspects of indigenous indian herb Trachyspermum ammi (L). Curr Tradit Med 2020; 6(3): 172-87.
Rana D, Bhatt A, Lal B. Ethnobotanical knowledge among the semi-pastoral Gujjar tribe in the high altitude (Adhwari’s) of Churah subdivision, district Chamba, Western Himalaya. J Ethnobiol Ethnomed 2019; 15(1): 10.
[] [PMID: 30744678]
Garbyal SS, Aggarwal KK, Babu CR. Traditionally used medicinal plants in Dharchula Himalayas of Pithoragarh district, Uttaranchal. Indian J Tradit Knowl 2005; 4(2): 199-207.
Rana CS, Tiwari JK, Dangwal LR, Gairola S. Faith herbal healer knowledge document of Nanda devi Biosphere reserve, Uttarakhand India. Indian J Tradit Knowl 2013; 12(2): 308-14.
Traditional herbal knowledge among the inhabitants: A case study in Urgam Valley of Chamoli Garhwal, Uttarakhand, India. Evid Based Complement Alternat Med 2019; 20195656925
[] [PMID: 31275412]
Balodi KN, Purohit MV, Shridhar V, Arunachalam K. Ethno-medicinal uses of various plants species among the Jaad Bhotiya Community of Uttarakhand, Western Himalaya. Ethno Med 2018; 12(3): 189-97.
Sharma PK, Chauhan NS, Lal B. Observations on the traditional phytotherapy among the inhabitants of Parvati valley in western Himalaya, India. J Ethnopharmacol 2004; 92(2-3): 167-76.
[] [PMID: 15137998]
Sharma P, Samant SS. Diversity, distribution and indigenous uses of medicinal plants in Parbati Valley of Kullu district in Himachal Pradesh, Northwestern Himalaya. Asian J of Adv Basic Sci 2014; 2(1): 77-98.
Chauhan. A. Ethno-medicine of Bhotia tribe in Mana village of Uttarakhand. Int J Sociol Anthropol 2014; 6(10): 296-304.
Singh A, Lal M, Samant SS. Diversity, indigenous uses and conservation prioritization of medicinal plants in Lahaul valley, proposed Cold Desert Biosphere Reserve, India. Int J Biodivers Sci & Manag 2009; 5(3): 132-54.
Arya D, Goel S, Joshi GC, Sharma OR, Sharma SK. Medico- ethno botanical practices among Bhotia Tribe of Kumaon Himalaya: A case study from Bageshwar District, Uttarkhand, India. J Drug Res Ayurvedic Sci 2018; 3(1): 43-7.
Mohanta R, Chauhan NPS. Anthropogenic threats and its conservation on Himalayan brown bear (Ursus arctos) habitat in Kugti wildlife sanctuary, Himachal Pradesh, India. Asian Journal of Conservation Biology 2014; 3(2): 175-80.
Radha PS. Study of wild medicinal plants used by tribal migratory shepherds in hills of shimla district, Himachal Pradesh. Plant Arch 2019; 19(1): 785-90.
Radha. Puri S. Pundir. A. Survey of wild medicinal plants used by migratory shepherds in summer hill of District Shimla in Himachal Pradesh. Bio Bulletin 2019; 5(1): 18-24.
Nayak BS, Patel KN. Pharmacognostic studies of the Jatropha curcas leaves. Int J Pharm Tech Res 2010; 2: 140-3.
Rao RS, Ramayya N. Trichome types and their taxonomic importance in the Tiliaceae. Indian J Bot 1987; 10: 65-73.

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Article Details

Year: 2020
Published on: 16 November, 2020
Page: [89 - 103]
Pages: 15
DOI: 10.2174/2211550109666200303111411
Price: $65

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