Title:Alpha Tocopherol Loaded in Liposome: Preparation, Optimization, Characterization and Sperm Motility Protection
VOLUME: 10 ISSUE: 3
Author(s):Lamia Taouzinet, Sofiane Fatmi*, Allaeddine Khellouf, Mohamed Skiba and Mokrane Iguer-ouada
Affiliation:Department of Processes Engineering, Faculty of Technology, Technology Pharmaceutical Laboratory, Université de Bejaia, 06000, Bejaia, Department of Processes Engineering, Faculty of Technology, Technology Pharmaceutical Laboratory, Université de Bejaia, 06000, Bejaia, Faculty of Nature and Life Sciences, Associated Laboratory in Marine Ecosystems and Aquaculture, Université de Bejaia, 06000, Bejaia, Technology Pharmaceutical and Bio pharmaceutics Laboratory, UFR Medicine and Pharmacy, Rouen University, 22 Blvd. Gambetta, 76183, Rouen, Faculty of Nature and Life Sciences, Associated Laboratory in Marine Ecosystems and Aquaculture, Université de Bejaia, 06000, Bejaia
Keywords:Experimental design, alpha tocopherol, cholesterol, liposome, morphology, sperm motility.
Abstract:Background: Alpha-tocopherol is a potent antioxidant involved in sperm protection particularly
during cryopreservation. However, its poor solubility limits the optimal protection in aqueous solutions.
Objective: The aim of this study was to enhance the solubility of α-tocopherol by the use of liposomes.
Methods: The experimental approach included loading vitamin E in liposomes prepared by ethanol injection
method and the optimization carried out by an experimental design. The optimum solution was
characterized by high performance liquid chromatography and scanning electron microscope. Finely, the
impact on sperm motility protection was studied by the freezing technic of bovine sperm.
Results: The optimum solution was obtained when using 10.9 mg/ml of phospholipids, 1.7 mg/ml of
cholesterol and 2 mg/ml of vitamin E. The liposome size was 99.86 nm, providing 78.47% of loaded efficiency.
The results showed a significant positive impact on sperm motility after hours of preservation.
Conclusion: In conclusion, the current results showed the interest of liposome preparation as an alternative
to enhance vitamin E solubility and to protect spermatozoa during cryopreservation.
