Alpha Tocopherol Loaded in Liposome: Preparation, Optimization, Characterization and Sperm Motility Protection

Author(s): Lamia Taouzinet, Sofiane Fatmi*, Allaeddine Khellouf, Mohamed Skiba, Mokrane Iguer-ouada

Journal Name: Drug Delivery Letters

Volume 10 , Issue 3 , 2020

DOI : 10.2174/2210303110666200302113209

  Journal Home
Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Background: Alpha-tocopherol is a potent antioxidant involved in sperm protection particularly during cryopreservation. However, its poor solubility limits the optimal protection in aqueous solutions.

Objective: The aim of this study was to enhance the solubility of α-tocopherol by the use of liposomes.

Methods: The experimental approach included loading vitamin E in liposomes prepared by ethanol injection method and the optimization carried out by an experimental design. The optimum solution was characterized by high performance liquid chromatography and scanning electron microscope. Finely, the impact on sperm motility protection was studied by the freezing technic of bovine sperm.

Results: The optimum solution was obtained when using 10.9 mg/ml of phospholipids, 1.7 mg/ml of cholesterol and 2 mg/ml of vitamin E. The liposome size was 99.86 nm, providing 78.47% of loaded efficiency. The results showed a significant positive impact on sperm motility after hours of preservation.

Conclusion: In conclusion, the current results showed the interest of liposome preparation as an alternative to enhance vitamin E solubility and to protect spermatozoa during cryopreservation.

Keywords: Experimental design, alpha tocopherol, cholesterol, liposome, morphology, sperm motility.

[1]
Duhem, N.; Danhier, F.; Préat, V. Vitamin E-based nanomedicines for anti-cancer drug delivery. J. Control. Release, 2014, 182, 33-44.
[http://dx.doi.org/10.1016/j.jconrel.2014.03.009] [PMID: 24631865]
[2]
Gonnet, M.; Lethuaut, L.; Boury, F. New trends in encapsulation of liposoluble vitamins. J. Control. Release, 2010, 146(3), 276-290.
[http://dx.doi.org/10.1016/j.jconrel.2010.01.037] [PMID: 20600399]
[3]
Gagné, A.; Wei, S.Q.; Fraser, W.D.; Julien, P. Absorption, transport, and bioavailability of vitamin e and its role in pregnant women. J. Obstet. Gynaecol. Can., 2009, 31(3), 210-217.
[http://dx.doi.org/10.1016/S1701-2163(16)34118-4] [PMID: 19416566]
[4]
Donnelly, E.T.; McClure, N.; Lewis, S.E.M. The effect of ascorbate and α-tocopherol supplementation in vitro on DNA integrity and hydrogen peroxide-induced DNA damage in human spermatozoa, mutage., 1999.
[http://dx.doi.org/10.1093/mutage/14.5.505]
[5]
Hezavehei, M.; Sharafi, M.; Kouchesfahani, H-M.; Henkel, R.; Agarwal, A.; Esmaeili, V.; Shahverdi, A. Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches. Reprod. Biomed. Online, 2018, 37(3), 327-339.
[http://dx.doi.org/10.1016/j.rbmo.2018.05.012] [PMID: 30143329]
[6]
Jeong, Y-J.; Kim, M-K.; Song, H-J.; Kang, E-J.; Ock, S-A.; Kumar, B.M.; Balasubramanian, S.; Rho, G-J. Effect of α-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes. Cryobiology, 2009, 58(2), 181-189.
[http://dx.doi.org/10.1016/j.cryobiol.2008.12.004] [PMID: 19141297]
[7]
Belala, R.; Fatmi, S.; Kaidi, R.; Iguer-Ouada, M. Benefits of cholesterol and α-tocopherol loaded,cyclodextrins in dog semen cryopreservation. Rev. Med. Vet., 2016, 1, 22-27.
[8]
Benhenia, K.; Rahab, H.; Smadi, M-A.; Benmakhlouf, H.; Lamara, A.; Idres, T.; Iguer-Ouada, M. Beneficial and harmful effects of cyclodextrin-vitamin E complex on cryopreserved ram sperm. Anim. Reprod. Sci., 2018, 195, 266-273.
[http://dx.doi.org/10.1016/j.anireprosci.2018.06.004] [PMID: 29891254]
[9]
Neunert, G.; Polewski, P.; Markiewicz, M.; Walejko, P.; Witkowski, S.; Polewski, K. Partition of tocopheryl glucopyranoside into liposome membranes studied by fluorescence methods. Biophys. Chem., 2010, 146(2-3), 92-97.
[http://dx.doi.org/10.1016/j.bpc.2009.10.009] [PMID: 19931243]
[10]
Fatmi, S.; Bournine, L.; Iguer-Ouada, M.; Lahiani-Skiba, M.; Bouchal, F.; Skiba, M. Amorphous solid dispersion studies of camptothecin-cyclodextrin inclusion complexes in PEG 6000. Acta Pol. Pharm., 2015, 72(1), 179-192.
[PMID: 25850214]
[11]
Uhumwangho, M.; Okor, R. Current trends in the production and biomedical applications of liposomes: a review J. Med. Biomed. Res., 2009, 4
[12]
Johnston, M-J-W.; Semple, S-C.; Klimuk, S-K.; Ansell, S.; Maurer, N.; Cullis, P-R. Characterization of the drug retention and pharmacokinetic properties of liposomal nanoparticles containing dihydrosphingomyelin. Biochim. Biophys. Acta, 2007, 1768(5), 1121-1127.
[http://dx.doi.org/10.1016/j.bbamem.2007.01.019] [PMID: 17321495]
[13]
Akbarzadeh, A.; Rezaei-Sadabady, R.; Davaran, S.; Joo, S.W.; Zarghami, N.; Hanifehpour, Y.; Samiei, M.; Kouhi, M.; Nejati-Koshki, K. 2013.
[14]
Laouini, A.; Jaafar-Maalej, C. Limayem- Blouza,I.; Sfar, S.; Charcosset, C.; and Fessi, H.; Preparation, characterization and applications of liposomes: state of the art, j. colloid sci. Biotechnol., 2012, 1, 147-168.
[15]
Sebaaly, C.; Jraij, A.; Fessi, H.; Charcosset, C.; Greige-Gerges, H. Preparation and characterization of clove essential oil-loaded liposomes. Food Chem., 2015, 178, 52-62.
[http://dx.doi.org/10.1016/j.foodchem.2015.01.067] [PMID: 25704683]
[16]
Gilbert, S.C. Virus-like particles as vaccine adjuvants. Mol. Biotechnol., 2001, 19(2), 169-177.
[http://dx.doi.org/10.1385/MB:19:2:169] [PMID: 11725486]
[17]
Costa, R.; Santos, L. Delivery systems for cosmetics - From manufacturing to the skin of natural antioxidants. Powder Technol., 2017, 322, 402-416.
[http://dx.doi.org/10.1016/j.powtec.2017.07.086]
[18]
Koudelka, S.; Turanek Knotigova, P.; Masek, J.; Prochazka, L.; Lukac, R.; Miller, A.D.; Neuzil, J.; Turanek, J. Liposomal delivery systems for anti-cancer analogues of vitamin E. J. Control. Release, 2015, 207, 59-69.
[http://dx.doi.org/10.1016/j.jconrel.2015.04.003] [PMID: 25861728]
[19]
Vijayakumar, M.R.; Vajanthri, K.Y.; Balavigneswaran, C.K.; Mahto, S.K.; Mishra, N.; Muthu, M.S.; Singh, S. Pharmacokinetics, biodistribution, in vitro cytotoxicity and biocompatibility of Vitamin E TPGS coated trans resveratrol liposomes. Colloids Surf. B Biointerfaces, 2016, 145, 479-491.
[http://dx.doi.org/10.1016/j.colsurfb.2016.05.037] [PMID: 27236510]
[20]
Laouini, A.; Charcosset, C.; Fessi, H.; Holdich, R.G. Preparation of liposomes: a novel application of microengineered membranes - investigation of the process parameters and application to the encapsulation of vitamin E RSC Adv., 2013, 3(15), 4985-4994.
[http://dx.doi.org/10.1039/C3RA23411H]
[21]
Padamwar, M.N.; Pokharkar, V.B. Development of vitamin loaded topical liposomal formulation using factorial design approach: drug deposition and stability. Int. J. Pharm., 2006, 320(1-2), 37-44.
[http://dx.doi.org/10.1016/j.ijpharm.2006.04.001] [PMID: 16707237]
[22]
Ma, Q.H.; Kuang, Y.Z.; Hao, X.Z.; Gu, N. Preparation and characterization of tea polyphenols and vitamin E loaded nanoscale complex liposome. J. Nanosci. Nanotechnol., 2009, 9(2), 1379-1383.
[http://dx.doi.org/10.1166/jnn.2009.C161] [PMID: 19441529]
[23]
Basiri, L.; Rajabzadeh, G.; Bostan, A. Physicochemical properties and release behavior of Span 60/Tween 60 niosomes as vehicle for α-Tocopherol delivery. LWT, 2017, 84, 471-478.
[http://dx.doi.org/10.1016/j.lwt.2017.06.009]
[24]
Betz, N.E.; Hammond, M.S.; Multon, K.D. Reliability and Validity of Five-Level Response Continua for the Career Decision Self-Efficacy Scale. J. Career Assess., 2005, 13, 131-149.
[http://dx.doi.org/10.1177/1069072704273123]
[25]
Priyanka, K.; Sahu, P.L.; Singh, S. Optimization of processing parameters for the development of Ficus religiosa L. extract loaded solid lipid nanoparticles using central composite design and evaluation of antidiabetic efficacy. J. Drug Deliv. Sci. Technol., 2018, 43, 94-102.
[http://dx.doi.org/10.1016/j.jddst.2017.08.006]
[26]
Kaur, G.; Mehta, S.K. Developments of polysorbate (Tween) based microemulsions: Preclinical drug delivery, toxicity and antimicrobial applications. Int. J. Pharm., 2017, 529(1-2), 134-160.
[http://dx.doi.org/10.1016/j.ijpharm.2017.06.059] [PMID: 28642203]
[27]
Patel, R.; Patel, H.; Baria, A. 2009.
[28]
Princely, S.; Dhanaraju, Md. Design, formulation, and characterization of liposomal-encapsulated gel for transdermal delivery of fluconazole. Asian J. Pharm. Clin. Res., 2018, 11, 417.
[http://dx.doi.org/10.22159/ajpcr.2018.v11i8.25621]
[29]
Baillie, A.J.; Florence, A.T.; Hume, L.R.; Muirhead, G.T.; Rogerson, A. The preparation and properties of niosomes--non-ionic surfactant vesicles. J. Pharm. Pharmacol., 1985, 37(12), 863-868.
[http://dx.doi.org/10.1111/j.2042-7158.1985.tb04990.x] [PMID: 2868092]
[30]
Monteiro, N.; Martins, A.; Reis, R-L.; Neves, N-M. Liposomes in tissue engineering and regenerative medicine; J. R So, 2014, p. 18.
[31]
Li, J.; Wang, X.; Zhang, T.; Wang, C.; Huang, Z.; Luo, X.; Deng, Y. A review on phospholipids and their main applications in drug delivery systems. Asian J. Pharm. Sci., 2015, 10, 81-98.
[http://dx.doi.org/10.1016/j.ajps.2014.09.004]
[32]
Khellouf, A.; Benhenia, K.; Fatmi, S.; Iguer-Ouada, M. The complementary effect of cholesterol and vitamin e preloaded in cyclodextrins on frozen bovine semen: motility parameters, membrane integrity and lipid peroxidation, cryoletters., 2018.
[33]
Belala, R.; Delay, J.; Amirat, L.; Ropers, M-H.; Guillou, J.L.; Anton, M.; Schmitt, E.; Thorin, C.; Michaud, S.; Kaidi, R.; Tainturier, D.; Bencharif, D. The benefits of liposomes for chilling canine sperm for 4 days at 4°C. Anim. Reprod. Sci., 2016, 168, 100-109.
[http://dx.doi.org/10.1016/j.anireprosci.2016.02.032] [PMID: 26952759]


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 10
ISSUE: 3
Year: 2020
Published on: 10 September, 2020
Page: [228 - 236]
Pages: 9
DOI: 10.2174/2210303110666200302113209
Price: $25

Article Metrics

PDF: 21
HTML: 2
EPUB: 1
PRC: 1



Most Downloaded Article(s)