Aims: The present study aims to determine the phototoxic and haemolytic activity of organophosphorus.
The use of alternative in vitro assays with human erythrocytes is suggested to predict the
polluting effect of these products on health.
Methodology: Human erythrocytes from Toluca Blood Bank were used. Sodium dodecyl sulfate was
employed as a positive control. Additionally, the haemolysis percentage of three organophosphate (Acetate,
Chlorpyrifos, Malathion, Methamidophos, Methyl Parathion) induced photo haemolysis formulated
with surfactants on a concentration of 2 x 109 erythrocytes were evaluated. Finally, the products were
classified as irritant or phototoxic.
Results: Results showed that the HC50 red blood cells were similar for each organophosphate
(Malathion and Methamidophos) indicating very irritant action with ratio classification (L/D) of 0.041
and 0.053, respectively. On the other hand, Chlorpyrifos was classified as an irritant with L/D= 0.14. On
the other hand, the HC50 obtained photo hemolysis assays irradiated red blood cells was similar for each
organophosphate (Acetate, Chlorpyrifos, Malathion, Methamidophos, Methyl Parathion) indicating no
Conclusion: As a conclusion, it can be said that the parameters of haemolysis and denaturation of proteins
are good indicators to classify organophosphorus formulated with surfactants as irritating or phototoxic.