Background: Alcohol withdrawal syndrome leads to irritability, aggressiveness, body
posture and motor abnormalities, sensory hyper reactivity and changes in various enzyme levels.
Dhatryadi ghrita penetrates the blood-brain barrier to decrease the cravings for alcohol in this syndrome.
Objective: To evaluate the effect of alcoholic extract of Dhatryadi ghrita on alcohol withdrawal
syndrome in Wistar rats.
Materials & Methods: A liquid diet with 7.2%, v/v ethanol was administered to the Wistar rats for
21 days. Control group animals received saline and normal diet. After alcohol withdrawal, rats
were examined at 6th and 24th hour for anxiety and hyper locomotor activity as major withdrawal
signs. Anxiety due to ethanol withdrawal was tested with the help of elevated plus maze, light and
dark models. The hyper locomotor activity was assessed using Actophotometer. The hepatic enzymes
level was determined with the help of the Bio-chemical Analyzer. ghrita extracts (100,
200,300 mg/kg, oral) were administered to different groups and diazepam as standard (2 mg/kg,
i.p) was administered to the treatment group animals 30 minutes before alcohol withdrawal estimation.
Drug treatment was administered 30 minutes before the second observation at the 24th hour.
Results: Findings from the present study revealed that Ghrita extract treatment at doses 100, 200
and 300 mg/kg, oral in ethanol-dependent rats had a significant protective effect on signs and symptoms
of ethanol withdrawal in alcohol-dependent rats.
Conclusion: Dhatryadi extract acts effectively for the treatment of alcohol abstinence syndrome.
The extract treatment has beneficial effects on ethanol withdrawal depressive-like behavior in rats.