Background: Meloxicam is a non-steroidal anti-inflammatory drug, known to inhibit cyclooxygenase,
a key enzyme of prostaglandin biosynthesis at the site of inflammation.
Objectives: Two sensitive and fast spectrophotometric methods (direct and indirect) using flOW INJection
Analysis (FIA) have been described for the assay of meloxicam in pure and in pharmaceutical
Methods: The direct method (method A) depended on the coupling of meloxicam with diazotized procaine
benzylpenicillin in alkaline medium. In contrast, the indirect method (method B) involved a
charge transfer reaction between the alkaline hydrolytic product of meloxicam as n-donor with metol
(p-methylaminophenol sulfate) as a π- acceptor using sodium periodate as an oxidant.
Results: The colored products exhibited absorption maxima at 492 and 656 nm for method A and B
respectively. The methods obeyed Beer’s low over concentration ranges of 5-80 and 15-225 μg/mL,
respectively. The detection limits were 2.73 and 5.26 μg/mL for direct and indirect methods, respectively.
The chemical and physical parameters associated with FIA manifolds were optimized to construct
the most sensitive and reproducible results.
Conclusion: Methods described herein are cheap, environmentally friendly and showed no significant
difference with those of the official method indicated no significant difference in accuracy and precision.