Aim: A new, accurate and sensitive reversed-phase high-performance liquid
chromatography (RP-HPLC) as an analytical method for the quantitative determination of eight
antifungal drugs in spiked human plasma has been described optimized and validated.
Materials and Methods: The analyzed compounds were voriconazole (VOR), luliconazole (LUL),
clotrimazole (CLO), tioconazole (TIO), posaconazole (POS), ketoconazole (KET), sertaconazole
(SER) and terconazole (TER).
Results: The separation of the analyzed compounds was conducted using a novel pentabromobenzyl
column known as COSMOSIL PBB-R (150 mm × 4.6 mm I.D., particle size 5 μm). The analysis of
the studied drugs was determined within 14 min using a diode array detector and the mobile phase
consisted of: 10 mM potassium dihydrogen phosphate buffer (pH 2.1): Methanol (2: 98 v/v). A linear
response was observed for all compounds in the range of concentration studied. Sample preparation
was done through liquid-liquid extraction using diethyl ether.
Conclusion: This proposed method was validated in terms of linearity, limit of quantification, limit
of detection, accuracy, precision and selectivity. The method was successfully applied for the
determination of these drugs in their pharmaceutical formulations and in human plasma samples.