Background: ADAMTS (A disintegrin-like metalloproteinase with thrombospondin
motifs) is a group of 19 zinc-dependent metalloproteases known to function in many pathological
and physiological processes, such as adhesion, cell fusion, signaling, proteolysis and ECM
Objectives: The aim of this study was to demonstrate the levels of ADAMTS-4 and -5 in gingival
tissues with Stage III-Grade B generalized periodontitis (SIII-GB), Stage III-Grade C generalized
periodontitis (SIII-GC) and healthy-control (C) groups.
Methods: The clinical measurements were recorded for each patient. A total of 63 gingival biopsy
specimens were obtained from the C (n:20), SIII-GB (n:23) and SIII-GC (n:20) groups.
Polymerase chain reaction (Rt-PCR) and immunohistochemical (IHC) examinations were used to
determine gene and protein levels.
Results: According to the results of all methods, ADAMTS-4 and -5 expressions existed in
periodontitis and C groups (P> 0.05). Immunostaining for ADAMTS-4 was found to be higher in
patients with periodontitis than for ADAMTS-5 (P>0.05). Gene expression levels for ADAMTS-4
and -5 seemed to be up-regulated in subjects diagnosed with periodontitis, but the results were not
statistically significant (P>0.05). A positive correlation was observed between PPD and ADAMTS-4
mRNA in SIII-GC (p=0.035) and SIII-GB (p=0.015). A positive correlation was determined between
ADAMTS-4 mRNA and ADAMTS-5 mRNA in SIII-GC (p=0.037) and SIII-GB (p=0.00).
Conclusion: ADAMTS expression may take part in both pathological and physiological processes
in the periodontal tissues, and periodontal destruction may be the result of a complex interaction of
several pathways with many participants, such as ADAMTS-4 and -5, thus facilitating the
exaggeration of periodontal disease.