Background and Objective: The present study was carried out to evaluate the anticancer property of
an alkylamino phenol derivative -2-((3,4-Dihydroquinolin-1(2H)-yl)(p-tolyl)methyl)phenol) (THTMP) against
human breast cancer cells. The cytotoxicity of the THTMP was assessed to know its specificity towards breast
cancer cells without affecting the normal cells.
Methods: The THTMP was synthesized and the cytotoxicity was assessed by MTT assay, Caspases enzyme
activity, DNA fragmentation and FITC/Annexin V, AO/EtBr staining, RT-PCR and QSAR. In addition, ADME
analysis was executed to understand the mode of action of THTMP.
Results: THTMP showed potential cytotoxic activity against the growth of MCF7 and SK-BR3 cells with the
IC50 values of 87.92μM and 172.51μM, respectively. Interestingly, THTMP found to activate caspase 3 and
caspase 9 enzymes in cancer cells, which are the key enzymes implicated in apoptosis. THTMP induced apoptosis
in which 33% of the cells entered the late apoptotic stage after 24h of treatment. The results also revealed
that the apoptotic response could be influenced by the association of THTMP with the Epidermal Growth Factor
Receptor (EGFR) mediated inhibition of the Phosphatidylinositol 3-Kinase (PI3K)/S6K1 signaling pathway. In
addition, docking was performed to study the binding mode of the THTMP, which shows better interaction with
EGFR. The structural elucidation of THTMP by Quantitative Structure-Activity Relationship model (QSAR)
and ADMET screening suggested, THTMP as an effective anticancer compound.
Conclusion: This work strengthens the potential of a promising drug-like compound, THTMP, for the discovery
of anticancer drug against breast cancer.