Background: Reduction in myocardial I/R injury has become the key to the therapy of
ischemic cardiovascular disease. Isoflurane (ISO) preconditioning can mimic the major potent protective
mechanisms and attenuate ischemia injury. Nevertheless, the mechanisms involved in the
cardioprotective effects afforded by isoflurane preconditioning have never been evaluated systematically.
Methods: Mice were randomly divided into an ISO preconditioning group and control group. The
size of the infarcted region was measured, and comparisons between ISO preconditioning and control
animals were made. The metabotropic glutamate receptor type 1(GRM1) expression levels in
all groups were determined by quantitative PCR. GRM1 protein expression and DNA damage relative
protein γ-H2AX were measured by western blot analysis. The oxidative stress was detected by
immunofluorescence after staining with the Dihydroethidium (DHE).
Results: ISO preconditioning significantly reduced the IR induced infarct volumes and reversed
the GRM1 protein expression level in I/R induced myocardial injury. Moreover, ISO preconditioning
has a protective effect in reducing the I/R induced DNA damage and oxidative stress.
Conclusion: The results of the present study have demonstrated that the expression of GRM1 provides
a protective role in ISO preconditioning against I/R-induced myocardial infarction by reducing
the oxidative stress and DNA damage.