Background: Liposomes are mostly known to be prepared from phospholipids and lipids and
have a remarkable capacity to encapsulate both lipophobic and lipophilic molecules. However, there is
little research on developing fatty acid liposomes for chemotherapy.
Objective: We have successfully prepared mixed fatty acid liposomes from two monounsaturated fatty
acids, namely oleic acid and erucic acid, which stabilised by DOPEPEG2000. The Critical Vesicular
Concentration (CVC) of liposomes was found to be within 0.09 to 0.21 mmol dm-3, with an average
particle size of 400 nm.
Methods: Encapsulation of various anticancer drugs such as folinic acid, methotrexate, doxorubicin, or
irinotecan resulted in Encapsulation Efficiency (%EE) of up to 90%. Using a 3-(4, 5-dimethylthiazol-2-
yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the median Inhibitory Concentration (IC50) values of
mixed oleic acid-erucic acid encapsulating hydrophilic drugs was remarkably reduced at the end of 24
hours of incubation with the human lung carcinoma cell line A549.
Results: The results suggest that mixed oleic acid-erucic acid liposomes are a potential new approach to
further develop as an alternative vehicle of various drugs for cancer treatment.