Novel polyheterocyclic nitrogen systems such as 2-amino-1H-pyrazolo[5',1':3,4][1,2,4]-
triazino[5,6-b]indole-3-carbonitrile 2, which has been synthesized for the first time in the present work,
is considered as starting material for the synthesis of more novel polyheterocyclic systems with five
fused rings and sometimes eight fused rings via heterocyclization with α,β-bifunctional reagents under
different conditions. The formed structures of the products were established from their correct elemental
analysis and spectral data. Some of the compounds were evaluated for their antimicrobial
activity using the Agar Well Diffusion method. Compounds 2, 4, 5, 6, 8 were found to be biologically
active (Supplementary information).