Background and Objectives: The present investigation is intended to prepare a Poly
Herbal Formulation (PHF) with Piper nigrum (fruits), Terminalia paniculata (bark) and Bauhinia
purpurea (bark) and assess their antioxidant and glucose-lowering effects utilizing in vitro models.
Methods: The individual plant methanolic extracts and PHF are exposed to phytochemical examination
and to distinguish the bioactive factors by GC-MS. We assessed the antioxidant properties of individual
plant extracts and the PHF by using the DPPH scavenging method, H2O2 scavenging assay,
TBARS assay and total antioxidant estimation. Likewise, the anti-diabetic activity was assessed by
ɑ-amylase and α-glucosidase enzyme inhibition and glucose diffusion inhibitory techniques.
Results: We found that PHF contains a high measure of total phenolics, total flavonoids and tannin
compared to individual plant extracts. The GC-MS identified the bioactive components. We also
found that PHF had significantly higher antioxidant and glucose-lowering effects than the individual
Conclusion: In conclusion, it could be reasoned that due to the nearness of antioxidant components,
the PHF has good potential in the administration of hyperglycemia, diabetes and the related state of
oxidative stress. This study shows that PHF is superior to individual plant extracts, supporting the
conventional PHF concept.