Background: This study determined the effect of Biochanin A (BCA) on isoproterenol
(ISO) induced Myocardial Infarction (MI) in male Wistar rats.
Methods: Animals (weighing 150-180 g) were divided into four groups, with six animals in each
group and pretreated with BCA (10mg/kg Body Weight [BW]) and ɑ-tocopherol (60mg/kg BW) for
30 days; and ISO (20mg/kg BW) was administrated subcutaneously on the 31st and 32nd day.
Results: ISO-induced MI rats demonstrated the significant elevation of serum glutamic oxaloacetic
transaminase, serum glutamic pyruvic transaminase, lactate dehydrogenase, creatine kinase-MB and
cardiac troponin; however, concomitant pretreatment with BCA protected the rats from cardiotoxicity
caused by ISO. Activities of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione
peroxidase, glutathione-S-transferase and glutathione reductase significantly reduced in the heart
with ISO-induced MI. Pretreatment with BCA produced a marked reversal of these antioxidant
enzymes related to MI-induced by ISO.
Conclusion: In conclusion, this study suggested that BCA exerts cardioprotective effects through
modulating lipid peroxidation, enhancing antioxidants, and detoxifying enzyme systems.