Screening of an Alkaline CMCase-Producing Strain and the Optimization of its Fermentation Condition

Author(s): Junmei Zhou, Lianghong Yin*, Chenbin Wu, Sijia Wu, Jidong Lu, Hailing Fang, Yongchang Qian*

Journal Name: Current Pharmaceutical Biotechnology

Volume 21 , Issue 13 , 2020


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Graphical Abstract:


Abstract:

Objective: Alkaline Carboxymethyl Cellulase (CMCase) is an attractive enzyme for the textile, laundry, pulp, and paper industries; however, commercial preparations with sufficient activity at alkaline conditions are scarce.

Methods: High CMCase-producing bacterial isolate, SX9-4, was screened out from soil bacteria, which was identified as Flavobacterium sp. on the basis of 16S rDNA sequencing.

Results: The optimum pH and temperature for CMCase reaction were 8.0 and 55°C, respectively. Alkaline CMCase was stable over wide pH (3.0-10.6) and temperature (25-55°C) ranges. Enzyme activity was significantly inhibited by the bivalent cations Mn2+ and Cu2+, and was activated by Fe2+. To improve the alkaline CMCase production of SX9-4, fermentation parameters were selected through onefactor- at-a-time and further carried out by response surface methodologies based on a central composite design.

Conclusion: High CMCase production (57.18 U/mL) was achieved under the optimal conditions: 10.53 g/L carboxymethylcellulose sodium, 7.74 g/L glucose, 13.71 g/L peptone, and 5.27 g/L ammonium oxalate.

Keywords: Alkaline CMCase, screening, identification, enzyme characteristics, optimization, fermentation.

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Article Details

VOLUME: 21
ISSUE: 13
Year: 2020
Published on: 28 November, 2020
Page: [1304 - 1315]
Pages: 12
DOI: 10.2174/1389201021666200129123818
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