Background: The rhizosphere microbiota are of vital importance for plant growth and
health in terrestrial ecosystems. There have been extensive studies aiming to identify the microbial
communities as well as their relationship with host plants in different soil types.
Objective: In the present study, we have employed the high-throughput sequencing technology to
investigate the composition and structure of rhizosphere microbiota prosperous at the root of
Dangshan Su pear growing in sandy soil and clay soil.
Methods: A high-throughput amplicon sequencing survey of the bacterial 16S rRNA genes and
fungal ITS regions from rhizosphere microbiota was firstly performed. Subsequently, several
common bacterial and fungal communities were found to be essential to Dangshan Su pear by using
a series of bioinformatics and statistics tools. Finally, the soil-preferred microbiota were identified
through variance analysis and further characterized in the genus level.
Result: Dangshan Su pears host rich and diverse microbial communities in thin layer of soil
adhering to their roots. The composition of dominant microbial phyla is similar across different soil
types, but the quantity of each microbial community varies significantly. Specially, the relative
abundance of Firmicutes increases from 9.69% to 61.66% as the soil ecosystem changes from clay
to sandy, which can be not only conducive to the degradation of complex plant materials, but also
responsible for the disinfestation of pathogens.
Conclusion: Our results have a symbolic significance for the potential efforts of rhizosphere
microbiota on the soil bioavailability and plant health. Through selecting soil types and altering
microbial structures, the improvement of fruit quality of Dangshan Su pear is expected to be