Background: Banisteriopsis campestris is a Malpighiaceae, also known as “cipó-prata” or
“murici”. There are some reports about the use of this plant in folk medicine.
Objectives: The aim of this study is to test the Essential Oils (EOs) from leaves, stems, and roots of B.
campestris for antibacterial, antifungal, antioxidant, and antiprotozoal activities and the inhibition of
glycation and cytotoxicity on Vero cells.
Methods: The plant was collected and the essential oil was obtained and tested for antibacterial, antifungal,
antioxidant, and antiprotozoal activities and the inhibition of glycation and cytotoxicity on Vero
cells, using the more adequate methods to achieve the objectives.
Results: The EOs inhibited the growth of aerobic and anaerobic oral bacteria. The root oil presented the
highest antibacterial activity with MIC levels ranging from 12.5 to 100 μg mL-1. The three EOs showed
antiprotozoal activity against Leishmania amazonensis. The stem and root EOs presented low cytotoxicity
to Vero cells. The roots and stem oils showed inhibition of glycation above 50%, with stem oil with
of 79.11%. The compounds identified in the leaf EOs were palmitic acid (22.98%), phytol (22.98%),
and triacontane (14.88%); in the stem–palmitic (49.79%), linoleic (11.63%), oleic (4.83%), and palmitoleic
(4.15%) fatty acids; in the root–palmitic acid (57.39%), linoleic (10.38%), and oleic acids
Conclusion: The results presented indicate that the EOs have remarkable antioxidant properties and
potential antimicrobial activity and confirm the essential oils of B. campestris as an alternative source of