Background: 6-Gingerol (6G) and 6-Shogaol (6S) are the main active components of ginger.
6-Gingerol is known for its anti-metastatic and anti-invasive pharmacological activities on cancer cells, besides,
6-Shogaol also inhibits breast cancer cell invasion.
Objective: In this study, radioiodination (131I) of 6G and 6S was aimed. Additionally, it is aimed to monitor their
incorporation behavior on breast cancer cell lines.
Methods: 6-Gingerol was isolated from the fresh ginger-roots extract, additionally, dehydrated to obtain
6-Shogaol. 6G and 6S were radioiodinated using iodogen method. Quality control studies of radioiodinated ginger
compounds (6G and 6S) were performed by thin layer radio-chromatography. In vitro studies of radioiodinated
ginger compounds on MCF-7 and MDA-MB-231 cells were performed with incorporation assays.
Results: 6-Gingerol and 6-Shogaol were radioiodinated (131I-6G and 131I-6S) in high yields over 95%. 131I-6S
demonstrated higher incorporation values than 131I-6G on MDA-MB-231 cells. Incorporation behavior of 131I-6G
and 131I-6S was similar to MCF-7 cells.
Conclusion: It has been observed that ginger compounds were radioiodinated successfully and 131I-6S have a
noteworthy incorporation on MDA-MB-231 cells which is a known breast carcinoma cell line with highly invasive