Title:Biodecomposition of Phenanthrene and Pyrene by a Genetically Engineered <i>Escherichia coli</i>
VOLUME: 14 ISSUE: 2
Author(s):Maryam Ahankoub , Gashtasb Mardani, Payam Ghasemi-Dehkordi*, Ameneh Mehri-Ghahfarrokhi, Abbas Doosti, Mohammad-Saeid Jami, Mehdi Allahbakhshian-Farsani, Javad Saffari-Chaleshtori and Mohammad Rahimi-Madiseh
Affiliation:Department of Geology, Payame Noor University, Tehran, Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Department of Hematology and Blood Banking, Faculty of Allied Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord
Keywords:Bioremediation, genetically engineered microorganisms, PAH, catechol 2, 3-dioxygenase,
nahH gene, HPLC.
Abstract:
Background: Genetically engineered microorganisms (GEMs) can be used for bioremediation
of the biological pollutants into nonhazardous or less-hazardous substances, at lower
cost. Polycyclic aromatic hydrocarbons (PAHs) are one of these contaminants that associated with
a risk of human cancer development. Genetically engineered E. coli that encoded catechol 2,3-
dioxygenase (C230) was created and investigated its ability to biodecomposition of phenanthrene
and pyrene in spiked soil using high-performance liquid chromatography (HPLC) measurement.
We revised patents documents relating to the use of GEMs for bioremediation. This approach
have already been done in others studies although using other genes codifying for same catechol
degradation approach.
Objective: In this study, we investigated biodecomposition of phenanthrene and pyrene by a genetically
engineered Escherichia coli.
Methods: Briefly, following the cloning of C230 gene (nahH) into pUC18 vector and transformation
into E. coli Top10F, the complementary tests, including catalase, oxidase and PCR were used
as on isolated bacteria from spiked soil.
Results: The results of HPLC measurement showed that in spiked soil containing engineered
E. coli, biodegradation of phenanthrene and pyrene comparing to autoclaved soil that inoculated
by wild type of E. coli and normal soil group with natural microbial flora, were statistically significant
(p<0.05). Moreover, catalase test was positive while the oxidase tests were negative.
Conclusion: These findings indicated that genetically manipulated E. coli can provide an effective
clean-up process on PAH compounds and it is useful for bioremediation of environmental pollution
with petrochemical products.
