Biodecomposition of Phenanthrene and Pyrene by a Genetically Engineered Escherichia coli

Author(s): Maryam Ahankoub, Gashtasb Mardani, Payam Ghasemi-Dehkordi*, Ameneh Mehri-Ghahfarrokhi, Abbas Doosti, Mohammad-Saeid Jami, Mehdi Allahbakhshian-Farsani, Javad Saffari-Chaleshtori, Mohammad Rahimi-Madiseh

Journal Name: Recent Patents on Biotechnology

Volume 14 , Issue 2 , 2020

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Abstract:

Background: Genetically engineered microorganisms (GEMs) can be used for bioremediation of the biological pollutants into nonhazardous or less-hazardous substances, at lower cost. Polycyclic aromatic hydrocarbons (PAHs) are one of these contaminants that associated with a risk of human cancer development. Genetically engineered E. coli that encoded catechol 2,3- dioxygenase (C230) was created and investigated its ability to biodecomposition of phenanthrene and pyrene in spiked soil using high-performance liquid chromatography (HPLC) measurement. We revised patents documents relating to the use of GEMs for bioremediation. This approach have already been done in others studies although using other genes codifying for same catechol degradation approach.

Objective: In this study, we investigated biodecomposition of phenanthrene and pyrene by a genetically engineered Escherichia coli.

Methods: Briefly, following the cloning of C230 gene (nahH) into pUC18 vector and transformation into E. coli Top10F, the complementary tests, including catalase, oxidase and PCR were used as on isolated bacteria from spiked soil.

Results: The results of HPLC measurement showed that in spiked soil containing engineered E. coli, biodegradation of phenanthrene and pyrene comparing to autoclaved soil that inoculated by wild type of E. coli and normal soil group with natural microbial flora, were statistically significant (p<0.05). Moreover, catalase test was positive while the oxidase tests were negative.

Conclusion: These findings indicated that genetically manipulated E. coli can provide an effective clean-up process on PAH compounds and it is useful for bioremediation of environmental pollution with petrochemical products.

Keywords: Bioremediation, genetically engineered microorganisms, PAH, catechol 2, 3-dioxygenase, nahH gene, HPLC.

[1]
Urgun-Demirtas M, Stark B, Pagilla K. Use of genetically engineered microorganisms (GEMs) for the bioremediation of contaminants. Crit Rev Biotechnol 2006; 26(3): 145-64.
[http://dx.doi.org/10.1080/07388550600842794] [PMID: 16923532]
[2]
Jain PK, Gupta VK, Gaur RK, Lowry M, Jaroli DP, Chauhan UK. Bioremediation of petroleum oil contaminated soil and water‏. Res J Environ Toxicol 2011; 5(1): 1-26.
[3]
Kalu AU. Bioremediation and environmental sustainability in Nigeria. Int J Acad Res Progress Educ Dev 2012; 1: 2226-6348.
[4]
Karigar CS, Rao SS. Role of microbial enzymes in the bioremediation of pollutants: a review. Enzyme Res 2011; 2011: 805187
[http://dx.doi.org/10.4061/2011/805187]
[5]
Das N, Chandran P. Microbial degradation of petroleum hydrocarbon contaminants: an overview. Biotechnol Res Int 2011; 2011: 941810
[http://dx.doi.org/10.4061/2011/941810]
[6]
Odonkor ST, Ampofo JK. Escherichia coli as an indicator of bacteriological quality of water: an overview. Microbiol Res (Pavia) 2013; 4: 2.
[http://dx.doi.org/10.4081/mr.2013.e2]
[7]
Percival SL, Yates MV, Williams D, Chalmers R, Gray N. Microbiology of waterborne diseases : microbiological aspects and risks. Cambridge, Massachusetts: Academic Press 2014.
[8]
Bélanger L, Garenaux A, Harel J, Boulianne M, Nadeau E, Dozois CM. Escherichia coli from animal reservoirs as a potential source of human extraintestinal pathogenic E. coli. FEMS Immunol Med Microbiol 2011; 62(1): 1-10.
[http://dx.doi.org/10.1111/j.1574-695X.2011.00797.x] [PMID: 21362060]
[9]
Winfield MD, Groisman EA. Role of nonhost environments in the lifestyles of Salmonella and Escherichia coli. Appl Environ Microbiol 2003; 69(7): 3687-94.
[http://dx.doi.org/10.1128/AEM.69.7.3687-3694.2003] [PMID: 12839733]
[10]
Reinthaler FF, Posch J, Feierl G, Wüst G, Haas D, Ruckenbauer G, et al. Antibiotic resistance of E. coli in sewage and sludge. Water Res 2003; 37(8): 1685-90.
[http://dx.doi.org/10.1016/S0043-1354(02)00569-9] [PMID: 12697213]
[11]
Castro-Silva C, Ruíz-Valdiviezo VM, Valenzuela-Encinas C, Alcántara-Hernández RJ. The bacterial community structure in an alkaline saline soil spiked with anthracene. Electron J Biotechnol 2013; 16: 1-20.
[12]
Mahvi A, Mardani G, Ghasemi-Dehkordi P, Saffari-Chaleshtori J, Hashemzadeh-Chaleshtori M, Allahbakhshian-Farsani M, et al. Effects of phenanthrene and pyrene on cytogenetic stability of human dermal fibroblasts using alkaline comet assay technique. Proc Natl Acad Sci, India, Sect B Biol Sci 2015; 85: 1055-63.
[http://dx.doi.org/10.1007/s40011-015-0514-0]
[13]
Arbabi M, Nasseri RI, Anyakora C. Simin. Biodegradation of polycyclic aromatic hydrocarbons (PAHs) in petroleum contaminated soils. IjcceAcIr 2009; 28: 53-9.
[14]
Mardani G, Mahvi AH, Hashemzadeh-Chaleshtori M, Nasseri S, Dehghani MH, Ghasemi-Dehkordi P. Degradation of phenanthrene and pyrene using genetically engineered dioxygenase producing Pseudomonas putida in soil. Genetika 2016; 48: 837-58.
[http://dx.doi.org/10.2298/GENSR1603837M]
[15]
Yu H. Environmental carcinogenic polycyclic aromatic hydrocarbons: Photochemistry and phototoxicity. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 2002; 20(2): 149-83.
[http://dx.doi.org/10.1081/GNC-120016203] [PMID: 12515673]
[16]
Mahvi AH, Mardani G. Determination of Phenanthrene in urban runoff of tehran, capital of Iran. Iran J Environ Health Sci Eng 2005; 2: 5-11.
[17]
Almeda R, Wambaugh Z, Chai C, Wang Z, Liu Z, Buskey EJ. Effects of crude oil exposure on bioaccumulation of polycyclic aromatic hydrocarbons and survival of adult and larval stages of gelatinous zooplankton. PLoS One 2013; 8(10): e74476
[http://dx.doi.org/10.1371/journal.pone.0074476] [PMID: 24116004]
[18]
Kasuga I, Nakajima F, Furumai H. Diversity of catechol 2,3-dioxygenase genes of bacteria responding to dissolved organic matter derived from different sources in a eutrophic lake. FEMS Microbiol Ecol 2007; 61(3): 449-58.
[http://dx.doi.org/10.1111/j.1574-6941.2007.00347.x] [PMID: 17645532]
[19]
Okuta A, Ohnishi K, Harayama S. Construction of chimeric catechol 2,3-dioxygenase exhibiting improved activity against the suicide inhibitor 4-methylcatechol. Appl Environ Microbiol 2004; 70(3): 1804-10.
[http://dx.doi.org/10.1128/AEM.70.3.1804-1810.2004] [PMID: 15006807]
[20]
Zhao H-P, Liang S-H, Yang X. Isolation and characterization of catechol 2,3-dioxygenase genes from phenanthrene degraders Sphingomonas, sp. ZP1 and Pseudomonas sp. ZP2. Environ Technol 2011; 33(15-16): 1895-901.
[http://dx.doi.org/10.1080/09593330.2011.568007] [PMID: 22439578]
[21]
Guo G, Fang T, Wang C, Huang Y, Tian F, Cui Q, et al. Isolation and characterization of two novel halotolerant Catechol 2, 3-dioxygenases from a halophilic bacterial consortium. Sci Rep 2015; 5: 17603.
[http://dx.doi.org/10.1038/srep17603] [PMID: 26621792]
[22]
Kanaly RA, Harayama S. Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by bacteria. J Bacteriol 2000; 182(8): 2059-67.
[http://dx.doi.org/10.1128/JB.182.8.2059-2067.2000] [PMID: 10735846]
[23]
Molecular cloning: a laboratory manual / Joseph Sambrook, David W. Russell - Details - Trove. Available at: https://trove.nla.gov.au/work/13615226 Accessed January 16, 2020.
[24]
Kobayashi T, Ishida T, Horiike K, Takahara Y, Numao N, Nakazawa A, et al. Overexpression of Pseudomonas putida catechol 2,3-dioxygenase with high specific activity by genetically engineered Escherichia coli. J Biochem 1995; 117(3): 614-22.
[http://dx.doi.org/10.1093/oxfordjournals.jbchem.a124753] [PMID: 7629031]
[25]
Neidle EL, Ornston LN. Cloning and expression of Acinetobacter calcoaceticus catechol 1,2-dioxygenase structural gene catA in Escherichia coli. J Bacteriol 1986; 168(2): 815-20.
[http://dx.doi.org/10.1128/JB.168.2.815-820.1986] [PMID: 3536862]
[26]
Fujita M, Kamiya T, Ike M, Kawagoshi Y, Shinohara N. Catechol 2,3-oxygenase production by genetically engineered Escherichia coli and its application to catechol determination. World J Microbiol Biotechnol 1991; 7(3): 407-14.
[http://dx.doi.org/10.1007/BF00329409] [PMID: 24425030]
[27]
Parales RE, Ontl TA, Gibson DT. Cloning and sequence analysis of a catechol 2,3-dioxygenase gene from the nitrobenzene-degrading strain Comamonas sp JS765. J Ind Microbiol Biotechnol 1997; 19(5-6): 385-91.
[http://dx.doi.org/10.1038/sj.jim.2900420] [PMID: 9451836]
[28]
Bordoloi J, Boruah HPD. Analysis of recent patenting activities in the field of bioremediation of petroleum hydrocarbon pollutants present in the environment. Recent Pat Biotechnol 2017; 12(1): 3-20.
[29]
Banerjee A, Roy A, Dutta S, Mondal S. Bioremediation of hydrocarbon-a review. Int J Adv Res 2016; 4(6): 1303-13.
[30]
Gupta G, Kumar V, Pal AK. Microbial degradation of high molecular weight polycyclic aromatic hydrocarbons with emphasis on pyrene. Polycycl Aromat Compd 2019; 39: 124-38.
[http://dx.doi.org/10.1080/10406638.2017.1293696]
[31]
Gilbert ES, Walker AW, Keasling JD. A constructed microbial consortium for biodegradation of the organophosphorus insecticide parathion. Appl Microbiol Biotechnol 2003; 61(1): 77-81.
[http://dx.doi.org/10.1007/s00253-002-1203-5] [PMID: 12658518]


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VOLUME: 14
ISSUE: 2
Year: 2020
Page: [121 - 133]
Pages: 13
DOI: 10.2174/1872208314666200128103513
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