Title:Coexistence of Metallo Beta Lactamase Resistant Gene Variants Among Clinical Isolates in Tertiary Care Hospital
VOLUME: 18
Author(s):Santhiya K., Jayanthi S*, Ananthasubramanian M. and Appalaraju B.
Affiliation:PSG College of Technology, Biotechnology, PSG College of Technology, Biotechnology, PSG College of Technology, Biotechnology, PSG Institute of Medical Science and Research, Microbiology
Keywords:Clinical isolates, carbapenem resistance, enterobacteriaceae, metallo beta lactamase, multidrug resistance, MBL
variants.
Abstract:Background: Carbapenem-resistant Enterobacteriaceae (CRE) has emerged as a global
threat with mortality risk ranging from 48%–71% worldwide. The emergence of MBL resistance is
threatening as carbapenem is one of the last line antibiotics. A total 24 variants of NDM resistance
raises a concern to the clinicians and epidemiologists worldwide.
Objective: The study aims at identifying MBL resistance (NDM, IMP, VIM, GIM, SPM, and SIM)
and its coexistence in clinical isolates in a single tertiary care center.
Methodology: Forty five clinical isolates characterized phenotypically for Carbapenem resistance
obtained from PSG Institute of Medical Science and Research (PSG IMSR), Coimbatore, between
February to March 2018 were taken for analysis.
Result: Out of the 45 Clinical isolates, 38 isolates (84%) were detected as MBL carriers. VIM,
NDM, GIM, and SPM were the predominant resistance genes, with detection rates of 48.8%,
28.8%, 24.4%, and 22.2% respectively. Fifteen isolates were observed to harbor more than one
MBL gene in coexistence. Two isolates - U42 and R714 (K. pneumoniae) were found to harbor all
5 MBL variants in combination.
Conclusion: 33% of clinical isolates harboring multiple MBL variants is a concern in clinical settings.
The presence of SPM and GIM gene amongst isolates in this geographical location within
India is an indicator demanding continuous monitoring of these resistance determinants.
